Human AGT ELISA Kit

Alternative name
Human serpin A8 ELISA Kit;Human Angiotensin 1-10 ELISA Kit;Human angiotensin I ELISA Kit;Human Ang I ELISA Kit;Human Angiotensin 1-8 ELISA Kit;Human angiotensin II ELISA Kit;Human Ang II ELISA Kit;Human Angiotensin 2-8 ELISA Kit;Human Angiotensin III ELISA Kit;Human Ang III ELISA Kit;Human Des-Asp[1]-angiotensin II ELISA Kit;Human Angiotensin 3-8 ELISA Kit;Human Angiotensin IV ELISA Kit;Human Ang IV ELISA Kit;Human SERPINA8 ELISA Kit;Human ANHU ELISA Kit;Human angiotensinogen ELISA Kit;Human alpha-1 antiproteinase, antitrypsin ELISA Kit;Human pre-angiotensinogen ELISA Kit;Human serine (or cysteine) proteinase inhibitor ELISA Kit;Human serpin peptidase inhibitor, clade A, member 8 ELISA Kit;
Catalog
E002195
species
Human
GeneAGT
Standard Curve
Other Species Mouse Agt ELISA Kit Bovine AGT ELISA Kit Chicken AGT ELISA Kit Horse AGT ELISA Kit Rat Agt ELISA Kit Sheep AGT ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of AGT. No significant cross-reactivity or interference between AGT and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between AGT and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman AGT ELISA Kit allows for the in vitro quantitative determination of AGT , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyCardiovascular
Product Description
specificalPrinciple of the assay: AGT ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-AGT antibody and an AGT-HRP conjugate. The assay sample and buffer are incubated together with AGT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AGT concentration since AGT from samples and AGT-HRP conjugate compete for the anti-AGT antibody binding site. Since the number of sites is limited, as more sites are occupied by AGT from the sample, fewer sites are left to bind AGT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AGT concentration in each sample is interpolated from this standard curve.
Human Angiotensin 1-4 Protein information
Uniprot ID ANGT_HUMAN
Uniprot AC P01019; Q16358; Q16359; Q96F91;
UniGene Hs.19383;
GeneID 183
KEGG hsa:183;
Human Angiotensin 1-4 Protein SEQUENCE
SEQUENCE 485 AA; 53154 MW; 5026C2DFB2DD236E CRC64;
MRKRAPQSEM APAGVSLRAT ILCLLAWAGL AAGDRVYIHP FHLVIHNEST
CEQLAKANAG KPKDPTFIPA PIQAKTSPVD EKALQDQLVL VAAKLDTEDK
LRAAMVGMLA NFLGFRIYGM HSELWGVVHG ATVLSPTAVF GTLASLYLGA
LDHTADRLQA ILGVPWKDKN CTSRLDAHKV LSALQAVQGL LVAQGRADSQ
AQLLLSTVVG VFTAPGLHLK QPFVQGLALY TPVVLPRSLD FTELDVAAEK
IDRFMQAVTG WKTGCSLMGA SVDSTLAFNT YVHFQGKMKG FSLLAEPQEF
WVDNSTSVSV PMLSGMGTFQ HWSDIQDNFS VTQVPFTESA CLLLIQPHYA
SDLDKVEGLT FQQNSLNWMK KLSPRTIHLT MPQLVLQGSY DLQDLLAQAE
LPAILHTELN LQKLSNDRIR VGEVLNSIFF ELEADEREPT ESTQQLNKPE
VLEVTLNRPF LFAVYDQSAT ALHFLGRVAN PLSTA
UCSC uc001hty.6; human.;

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Human AGT ELISA Kit