Human SMPD1 ELISA Kit

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  • Alternative name

    Human acid sphingomyelinase ELISA Kit;Human ASMASE ELISA Kit;Human ASM ELISA Kit;Human NPD ELISA Kit;Human sphingomyelin phosphodiesterase 1 ELISA Kit;Human sphingomyelin phosphodiesterase ELISA Kit;Human sphingomyelin phosphodiesterase 1, acid lysosomal ELISA Kit;

  • Catalog
    E036424
  • species
    Human
  • GeneSMPD1
  • Other Species Mouse Smpd1 ELISA KitBovine SMPD1 ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity<0.469ng/ml
  • Intended UseHuman SMPD1 ELISA Kit allows for the in vitro quantitative determination of SMPD1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-ASM antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-ASM antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the ASM amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of ASM can be calculated.
  • Human Sphingomyelin phosphodiesterase Protein information
  • Uniprot ID ASM_HUMAN
  • Uniprot AC P17405; A8K8M3; E9PKS3; P17406; Q13811; Q16837; Q16841;
  • UniGene Hs.498173;
  • GeneID 6609
  • KEGG hsa:6609;
  • Human Sphingomyelin phosphodiesterase Protein SEQUENCE
  • SEQUENCE 629 AA; 69752 MW; C9888CB8359C42AC CRC64;

    MPRYGASLRQ SCPRSGREQG QDGTAGAPGL LWMGLVLALA LALALALSDS

    RVLWAPAEAH PLSPQGHPAR LHRIVPRLRD VFGWGNLTCP ICKGLFTAIN

    LGLKKEPNVA RVGSVAIKLC NLLKIAPPAV CQSIVHLFED DMVEVWRRSV

    LSPSEACGLL LGSTCGHWDI FSSWNISLPT VPKPPPKPPS PPAPGAPVSR

    ILFLTDLHWD HDYLEGTDPD CADPLCCRRG SGLPPASRPG AGYWGEYSKC

    DLPLRTLESL LSGLGPAGPF DMVYWTGDIP AHDVWHQTRQ DQLRALTTVT

    ALVRKFLGPV PVYPAVGNHE STPVNSFPPP FIEGNHSSRW LYEAMAKAWE

    PWLPAEALRT LRIGGFYALS PYPGLRLISL NMNFCSRENF WLLINSTDPA

    GQLQWLVGEL QAAEDRGDKV HIIGHIPPGH CLKSWSWNYY RIVARYENTL

    AAQFFGHTHV DEFEVFYDEE TLSRPLAVAF LAPSATTYIG LNPGYRVYQI

    DGNYSGSSHV VLDHETYILN LTQANIPGAI PHWQLLYRAR ETYGLPNTLP

    TAWHNLVYRM RGDMQLFQTF WFLYHKGHPP SEPCGTPCRL ATLCAQLSAR

    ADSPALCRHL MPDGSLPEAQ SLWPRPLFC

  • UCSC uc001mcw.4; human. [P17405-1];


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