SpecificityThe cross-reactivity of the triiodothyronine antibody to selected substances was evaluated by adding the interfering substance to a serum matrix at various concentrations. The cross-reactivity was calculated by deriving a ration between dose of interfering substance to dose of Triiodothyronine needed to displace the same amount of tracer.
SensitivityThe fT3 EIA procedure has a sensitivity of 0.05 pg/ml. The sensitivity was ascertained by determining the variability of the 0 pg/ml serum calibrator and using the 2? (95% certainty) statistic to calculate the minimum dose.
Intended UseHuman THVgp003 ELISA Kit allows for the in vitro quantitative determination of THVgp003 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalPrinciple of the assay: The fT3 test is a solid phase competitive enzyme immunoassay. Patient serum samples, standards, and T3-Enzyme Conjugate Working Reagent is added to wells coated with monoclonal T3 antibody. fT3 in the patient specimen and the T3 labeled conjugate compete for available binding sites on the antibody. After a 60 minutes incubation at room temperature, the wells are washed with water to remove unbound T3 conjugate. A solution of H2O2/TMB is then added and incubated for 20 minutes, resulting in the development of blue color. The color development is stopped with the addition of 3N HCl, and the absorbance is measured spectrophotometrically at 450 nm. The intensity of the color formed is proportional to the amount of enzyme present and is inversely related to the amount of unlabeled fT3 in the sample. By reference to a series of fT3 standards assayed in the same way, the concentration of fT3 in the unknown sample is quantified.