Human SIPA1 ELISA Kit

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  • Alternative name

    Human sipa-1 ELISA Kit;Human GTPase-activating protein Spa-1 ELISA Kit;Human p130 SPA-1 ELISA Kit;Human SPA1 ELISA Kit;Human signal-induced proliferation-associated 1 ELISA Kit;Human signal-induced proliferation-associated protein 1 ELISA Kit;Human signal-induced proliferation-associated gene 1 ELISA Kit;

  • Catalog
    E035300
  • species
    Human
  • GeneSIPA1
  • Standard CurveHuman SIPA1 ELISA Kit
  • Other Species Mouse Sipa1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of human SIPA1. No significant cross-reactivity or interference between human SIPA1 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human SIPA1 is typically less than 6.25 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mea
  • Intended UseHuman SIPA1 ELISA Kit allows for the in vitro quantitative determination of SIPA1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SIPA1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SIPA1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SIPA1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SIPA1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human Signal-induced proliferation-associated protein 1 Protein information
  • Uniprot ID SIPA1_HUMAN
  • Uniprot AC Q96FS4; O14518; O60484; O60618; Q2YD83;
  • UniGene Hs.530477;
  • GeneID 6494
  • KEGG hsa:6494;
  • Human Signal-induced proliferation-associated protein 1 Protein SEQUENCE
  • SEQUENCE 1042 AA; 112149 MW; FB52C7231EA3B6B9 CRC64;

    MPMWAGGVGS PRRGMAPAST DDLFARKLRQ PARPPLTPHT FEPRPVRGPL

    LRSGSDAGEA RPPTPASPRA RAHSHEEASR PAATSTRLFT DPLALLGLPA

    EEPEPAFPPV LEPRWFAHYD VQSLLFDWAP RSQGMGSHSE ASSGTLASAE

    DQAASSDLLH GAPGFVCELG GEGELGLGGP ASPPVPPALP NAAVSILEEP

    QNRTSAYSLE HADLGAGYYR KYFYGKEHQN FFGMDESLGP VAVSLRREEK

    EGSGGGTLHS YRVIVRTTQL RTLRGTISED ALPPGPPRGL SPRKLLEHVA

    PQLSPSCLRL GSASPKVPRT LLTLDEQVLS FQRKVGILYC RAGQGSEEEM

    YNNQEAGPAF MQFLTLLGDV VRLKGFESYR AQLDTKTDST GTHSLYTTYQ

    DHEIMFHVST MLPYTPNNQQ QLLRKRHIGN DIVTIVFQEP GSKPFCPTTI

    RSHFQHVFLV VRAHTPCTPH TTYRVAVSRT QDTPAFGPAL PAGGGPFAAN

    ADFRAFLLAK ALNGEQAAGH ARQFHAMATR TRQQYLQDLA TNEVTTTSLD

    SASRFGLPSL GGRRRAAPRG PGAELQAAGS LVWGVRAAPG ARVAAGAQAS

    GPEGIEVPCL LGISAEALVL VAPRDGRVVF NCACRDVLAW TFSEQQLDLY

    HGRGEAITLR FDGSPGQAVG EVVARLQLVS RGCETRELAL PRDGQGRLGF

    EVDAEGFVTH VERFTFAETA GLRPGARLLR VCGQTLPSLR PEAAAQLLRS

    APKVCVTVLP PDESGRPRRS FSELYTLSLQ EPSRRGAPDP VQDEVQGVTL

    LPTTKQLLHL CLQDGGSPPG PGDLAEERTE FLHSQNSLSP RSSLSDEAPV

    LPNTTPDLLL ATTAKPSVPS ADSETPLTQD RPGSPSGSED KGNPAPELRA

    SFLPRTLSLR NSISRIMSEA GSGTLEDEWQ AISEIASTCN TILESLSREG

    QPIPESGDPK GTPKSDAEPE PGNLSEKVSH LESMLRKLQE DLQKEKADRA

    ALEEEVRSLR HNNRRLQAES ESAATRLLLA SKQLGSPTAD LA

  • UCSC uc001ofb.3; human.;


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