Human CCND2 ELISA Kit

Alternative name
Human KIAK0002 ELISA Kit;Human MPPH3 ELISA Kit;Human cyclin D2 ELISA Kit;Human G1/S-specific cyclin-D2 ELISA Kit;
Catalog
E010539
species
Human
GeneCCND2
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman CCND2 ELISA Kit allows for the in vitro quantitative determination of CCND2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyCell Biology
Product Description
specificalPrinciple of the Assay: CCND2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CCND2 antibody and an CCND2-HRP conjugate. The assay sample and buffer are incubated together with CCND2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CCND2 concentration since CCND2 from samples and CCND2-HRP conjugate compete for the anti-CCND2 antibody binding site. Since the number of sites is limited, as more sites are occupied by CCND2 from the sample, fewer sites are left to bind CCND2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CCND2 concentration in each sample is interpolated from this standard curve.
Human G1/S-specific cyclin-D2 Protein information
Uniprot ID CCND2_HUMAN
Uniprot AC P30279; A8K531; Q13955; Q5U035;
UniGene Hs.376071;
GeneID 894
KEGG hsa:894;
Human G1/S-specific cyclin-D2 Protein SEQUENCE
SEQUENCE 289 AA; 33067 MW; E4E5FEF476D76D90 CRC64;
MELLCHEVDP VRRAVRDRNL LRDDRVLQNL LTIEERYLPQ CSYFKCVQKD
IQPYMRRMVA TWMLEVCEEQ KCEEEVFPLA MNYLDRFLAG VPTPKSHLQL
LGAVCMFLAS KLKETSPLTA EKLCIYTDNS IKPQELLEWE LVVLGKLKWN
LAAVTPHDFI EHILRKLPQQ REKLSLIRKH AQTFIALCAT DFKFAMYPPS
MIATGSVGAA ICGLQQDEEV SSLTCDALTE LLAKITNTDV DCLKACQEQI
EAVLLNSLQQ YRQDQRDGSK SEDELDQAST PTDVRDIDL
UCSC uc001qmo.4; human. [P30279-1];

Category

Human CCND2 ELISA Kit

Human KIAK0002 ELISA Kit;Human MPPH3 ELISA Kit;Human cyclin D2 ELISA Kit;Human G1/S-specific cyclin-D2 ELISA Kit;