Human CCND2 ELISA Kit

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  • Alternative name

    Human KIAK0002 ELISA Kit;Human MPPH3 ELISA Kit;Human cyclin D2 ELISA Kit;Human G1/S-specific cyclin-D2 ELISA Kit;

  • Catalog
    E010539
  • species
    Human
  • GeneCCND2
  • Standard CurveHuman CCND2 ELISA Kit
  • Other Species Mouse Ccnd2 ELISA KitBovine CCND2 ELISA KitChicken CCND2 ELISA KitPorcine CCND2 ELISA KitRat Ccnd2 ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman CCND2 ELISA Kit allows for the in vitro quantitative determination of CCND2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyCell Biology
  • Product Description
    specifical
    Principle of the Assay: CCND2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CCND2 antibody and an CCND2-HRP conjugate. The assay sample and buffer are incubated together with CCND2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CCND2 concentration since CCND2 from samples and CCND2-HRP conjugate compete for the anti-CCND2 antibody binding site. Since the number of sites is limited, as more sites are occupied by CCND2 from the sample, fewer sites are left to bind CCND2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CCND2 concentration in each sample is interpolated from this standard curve.
  • Human G1/S-specific cyclin-D2 Protein information
  • Uniprot ID CCND2_HUMAN
  • Uniprot AC P30279; A8K531; Q13955; Q5U035;
  • UniGene Hs.376071;
  • GeneID 894
  • KEGG hsa:894;
  • Human G1/S-specific cyclin-D2 Protein SEQUENCE
  • SEQUENCE 289 AA; 33067 MW; E4E5FEF476D76D90 CRC64;

    MELLCHEVDP VRRAVRDRNL LRDDRVLQNL LTIEERYLPQ CSYFKCVQKD

    IQPYMRRMVA TWMLEVCEEQ KCEEEVFPLA MNYLDRFLAG VPTPKSHLQL

    LGAVCMFLAS KLKETSPLTA EKLCIYTDNS IKPQELLEWE LVVLGKLKWN

    LAAVTPHDFI EHILRKLPQQ REKLSLIRKH AQTFIALCAT DFKFAMYPPS

    MIATGSVGAA ICGLQQDEEV SSLTCDALTE LLAKITNTDV DCLKACQEQI

    EAVLLNSLQQ YRQDQRDGSK SEDELDQAST PTDVRDIDL

  • UCSC uc001qmo.4; human. [P30279-1];


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