Human SIRPA ELISA Kit

Alternative name
Human SHP substrate 1 ELISA Kit;Human SHPS-1 ELISA Kit;Human Brain Ig-like molecule with tyrosine-based activation motifs ELISA Kit;Human BIT ELISA Kit;Human CD172 antigen-like family member A ELISA Kit;Human inhibitory receptor SHPS-1 ELISA Kit;Human macrophage fusion receptor ELISA Kit;Human myd-1 antigen ELISA Kit;Human Signal-regulatory protein alpha-1 ELISA Kit;Human Sirp-alpha-1 ELISA Kit;Human Signal-regulatory protein alpha-2 ELISA Kit;Human Sirp-alpha-2 ELISA Kit;Human Signal-regulatory protein alpha-3 ELISA Kit;Human Sirp-alpha-3 ELISA Kit;Human P84 ELISA Kit;Human MFR ELISA Kit;Human MYD1 ELISA Kit;Human PTPNS1 ELISA Kit;Human SHPS1 ELISA Kit;Human SIRP ELISA Kit;Human CD172A ELISA Kit;Human MYD-1 ELISA Kit;Human signal regulatory protein alpha ELISA Kit;Human tyrosine-protein phosphatase non-receptor type substrate 1 ELISA Kit;Human brain-immunoglobulin-like molecule with tyrosine-based activation motifs ELISA Kit;Human tyrosine phosphatase SHP substrate 1 ELISA Kit;
Catalog
E035245
species
Human
GeneSIRPA
SpecificityThis assay has high sensitivity and excellent specificity for detection of Signal Regulatory Protein Alpha (SIRPa). No significant cross-reactivity or interference between Signal Regulatory Protein Alpha (SIRPa) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.056ng/mL.
Intended UseHuman SIRPA ELISA Kit allows for the in vitro quantitative determination of SIRPA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Signal Regulatory Protein Alpha (SIRPa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Signal Regulatory Protein Alpha (SIRPa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Signal Regulatory Protein Alpha (SIRPa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Signal Regulatory Protein Alpha (SIRPa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Tyrosine-protein phosphatase non-receptor type substrate 1 Protein information
Uniprot ID SHPS1_HUMAN
Uniprot AC Q9H0Z2; Q9UDX2; Q9UIJ6; Q9Y4U9;
UniGene Hs.581021;
GeneID 140885
KEGG hsa:140885;
Human Tyrosine-protein phosphatase non-receptor type substrate 1 Protein SEQUENCE
SEQUENCE 504 AA; 54967 MW; 18D2FD04F6182AD0 CRC64;
MEPAGPAPGR LGPLLCLLLA ASCAWSGVAG EEELQVIQPD KSVLVAAGET
ATLRCTATSL IPVGPIQWFR GAGPGRELIY NQKEGHFPRV TTVSDLTKRN
NMDFSIRIGN ITPADAGTYY CVKFRKGSPD DVEFKSGAGT ELSVRAKPSA
PVVSGPAARA TPQHTVSFTC ESHGFSPRDI TLKWFKNGNE LSDFQTNVDP
VGESVSYSIH STAKVVLTRE DVHSQVICEV AHVTLQGDPL RGTANLSETI
RVPPTLEVTQ QPVRAENQVN VTCQVRKFYP QRLQLTWLEN GNVSRTETAS
TVTENKDGTY NWMSWLLVNV SAHRDDVKLT CQVEHDGQPA VSKSHDLKVS
AHPKEQGSNT AAENTGSNER NIYIVVGVVC TLLVALLMAA LYLVRIRQKK
AQGSTSSTRL HEPEKNAREI TQDTNDITYA DLNLPKGKKP APQAAEPNNH
TEYASIQTSP QPASEDTLTY ADLDMVHLNR TPKQPAPKPE PSFSEYASVQ
VPRK
UCSC uc002wfq.3; human. [P78324-1];

Category

Human SIRPA ELISA Kit