Human HPGD ELISA Kit

Alternative name
Human 15-PGDH ELISA Kit;Human prostaglandin dehydrogenase 1 ELISA Kit;Human short chain dehydrogenase/reductase family 36C member 1 ELISA Kit;Human PGDH1 ELISA Kit;Human SDR36C1 ELISA Kit;Human PGDH ELISA Kit;Human PHOAR1 ELISA Kit;Human hydroxyprostaglandin dehydrogenase 15-(NAD) ELISA Kit;Human 15-hydroxyprostaglandin dehydrogenase [NAD(+)] ELISA Kit;Human NAD+-dependent 15-hydroxyprostaglandin dehydrogenase ELISA Kit;
Catalog
E041735
species
Human
GeneHPGD
SpecificityThis assay has high sensitivity and excellent specificity for detection of HPGD. No significant cross-reactivity or interference between HPGD and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between HPGD and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman HPGD ELISA Kit allows for the in vitro quantitative determination of HPGD , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalIntended Uses: This HPGD ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human HPGD. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||HPGD ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-HPGD antibody and an HPGD-HRP conjugate. The assay sample and buffer are incubated together with HPGD-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HPGD concentration since HPGD from samples and HPGD-HRP conjugate compete for the anti-HPGD antibody binding site. Since the number of sites is limited, as more sites are occupied by HPGD from the sample, fewer sites are left to bind HPGD-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HPGD concentration in each sample is interpolated from this standard curve.
Human 15-hydroxyprostaglandin dehydrogenase [NAD(+)] Protein information
Uniprot ID PGDH_HUMAN
Uniprot AC Q12998;
UniGene Hs.596913;
GeneID 3248
KEGG hsa:3248;
Human 15-hydroxyprostaglandin dehydrogenase [NAD(+)] Protein SEQUENCE
SEQUENCE 266 AA; 28977 MW; B860D2DE80E49514 CRC64;
MHVNGKVALV TGAAQGIGRA FAEALLLKGA KVALVDWNLE AGVQCKAALD
EQFEPQKTLF IQCDVADQQQ LRDTFRKVVD HFGRLDILVN NAGVNNEKNW
EKTLQINLVS VISGTYLGLD YMSKQNGGEG GIIINMSSLA GLMPVAQQPV
YCASKHGIVG FTRSAALAAN LMNSGVRLNA ICPGFVNTAI LESIEKEENM
GQYIEYKDHI KDMIKYYGIL DPPLIANGLI TLIEDDALNG AIMKITTSKG
IHFQDYDTTP FQAKTQ
UCSC uc003itu.3; human. [P15428-1];

Human HPGD ELISA Kit

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