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Human HPGD ELISA Kit

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  • Alternative name

    Human 15-PGDH ELISA Kit;Human prostaglandin dehydrogenase 1 ELISA Kit;Human short chain dehydrogenase/reductase family 36C member 1 ELISA Kit;Human PGDH1 ELISA Kit;Human SDR36C1 ELISA Kit;Human PGDH ELISA Kit;Human PHOAR1 ELISA Kit;Human hydroxyprostaglandin dehydrogenase 15-(NAD) ELISA Kit;Human 15-hydroxyprostaglandin dehydrogenase [NAD(+)] ELISA Kit;Human NAD+-dependent 15-hydroxyprostaglandin dehydrogenase ELISA Kit;
  • Catalog
    E041735
  • species
    Human
  • GeneHPGD
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of HPGD. No significant cross-reactivity or interference between HPGD and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between HPGD and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman HPGD ELISA Kit allows for the in vitro quantitative determination of HPGD , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Intended Uses: This HPGD ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human HPGD. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||HPGD ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-HPGD antibody and an HPGD-HRP conjugate. The assay sample and buffer are incubated together with HPGD-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HPGD concentration since HPGD from samples and HPGD-HRP conjugate compete for the anti-HPGD antibody binding site. Since the number of sites is limited, as more sites are occupied by HPGD from the sample, fewer sites are left to bind HPGD-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HPGD concentration in each sample is interpolated from this standard curve.
  • Human 15-hydroxyprostaglandin dehydrogenase [NAD(+)] Protein information
  • Uniprot ID PGDH_HUMAN
  • Uniprot AC Q12998;
  • UniGene Hs.596913;
  • GeneID 3248
  • KEGG hsa:3248;
  • Human 15-hydroxyprostaglandin dehydrogenase [NAD(+)] Protein SEQUENCE

  • SEQUENCE 266 AA; 28977 MW; B860D2DE80E49514 CRC64;

    MHVNGKVALV TGAAQGIGRA FAEALLLKGA KVALVDWNLE AGVQCKAALD

    EQFEPQKTLF IQCDVADQQQ LRDTFRKVVD HFGRLDILVN NAGVNNEKNW

    EKTLQINLVS VISGTYLGLD YMSKQNGGEG GIIINMSSLA GLMPVAQQPV

    YCASKHGIVG FTRSAALAAN LMNSGVRLNA ICPGFVNTAI LESIEKEENM

    GQYIEYKDHI KDMIKYYGIL DPPLIANGLI TLIEDDALNG AIMKITTSKG

    IHFQDYDTTP FQAKTQ

  • UCSC uc003itu.3; human. [P15428-1];



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