Human SIK1 ELISA Kit

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  • Alternative name

    Human Salt-inducible kinase 1 ELISA Kit;Human SIK-1 ELISA Kit;Human serine/threonine-protein kinase SNF1-like kinase 1 ELISA Kit;Human serine/threonine-protein kinase SNF1LK ELISA Kit;Human SIK ELISA Kit;Human SNF1LK ELISA Kit;Human MSK ELISA Kit;Human salt inducible kinase 1 ELISA Kit;Human serine/threonine-protein kinase SIK1 ELISA Kit;Human SNF1-like kinase ELISA Kit;Human myocardial SNF1-like kinase ELISA Kit;Human salt-inducible protein kinase 1 ELISA Kit;

  • Catalog
    E034827
  • species
    Human
  • GeneSIK1
  • Other Species Mouse Sik1 ELISA KitRat Sik1 ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity<0.094ng/ml
  • Intended UseHuman SIK1 ELISA Kit allows for the in vitro quantitative determination of SIK1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-SIK1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-SIK1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the SIK1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of SIK1 can be calculated.
  • Human Serine/threonine-protein kinase SIK1 Protein information
  • Uniprot ID SIK1_HUMAN
  • Uniprot AC P57059; A6NC84; Q5R2V5; Q6ZNL8; Q86YJ2;
  • UniGene Hs.282113; Hs.700423;
  • GeneID 150094
  • KEGG hsa:150094;
  • Human Serine/threonine-protein kinase SIK1 Protein SEQUENCE
  • SEQUENCE 783 AA; 84902 MW; D646123C0715DAAC CRC64;

    MVIMSEFSAD PAGQGQGQQK PLRVGFYDIE RTLGKGNFAV VKLARHRVTK

    TQVAIKIIDK TRLDSSNLEK IYREVQLMKL LNHPHIIKLY QVMETKDMLY

    IVTEFAKNGE MFDYLTSNGH LSENEARKKF WQILSAVEYC HDHHIVHRDL

    KTENLLLDGN MDIKLADFGF GNFYKSGEPL STWCGSPPYA APEVFEGKEY

    EGPQLDIWSL GVVLYVLVCG SLPFDGPNLP TLRQRVLEGR FRIPFFMSQD

    CESLIRRMLV VDPARRITIA QIRQHRWMRA EPCLPGPACP AFSAHSYTSN

    LGDYDEQALG IMQTLGVDRQ RTVESLQNSS YNHFAAIYYL LLERLKEYRN

    AQCARPGPAR QPRPRSSDLS GLEVPQEGLS TDPFRPALLC PQPQTLVQSV

    LQAEMDCELQ SSLQWPLFFP VDASCSGVFR PRPVSPSSLL DTAISEEARQ

    GPGLEEEQDT QESLPSSTGR RHTLAEVSTR LSPLTAPCIV VSPSTTASPA

    EGTSSDSCLT FSASKSPAGL SGTPATQGLL GACSPVRLAS PFLGSQSATP

    VLQAQGGLGG AVLLPVSFQE GRRASDTSLT QGLKAFRQQL RKTTRTKGFL

    GLNKIKGLAR QVCQAPASRA SRGGLSPFHA PAQSPGLHGG AAGSREGWSL

    LEEVLEQQRL LQLQHHPAAA PGCSQAPQPA PAPFVIAPCD GPGAAPLPST

    LLTSGLPLLP PPLLQTGASP VASAAQLLDT HLHIGTGPTA LPAVPPPRLA

    RLAPGCEPLG LLQGDCEMED LMPCSLGTFV LVQ

  • UCSC uc002zdf.4; human.;


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