SpecificityThis assay has high sensitivity and excellent specificity for detection of Human NF-kappaB. No significant cross-reactivity or interference between Human NF-kappaB and analogues was observed.
Sensitivity0.156 ng/mL (mean of 6 independent assays)
Intended UseHuman NF-kappaB ELISA Kit allows for the in vitro quantitative determination of NF-kappaB , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalIntended Uses: For the quantitative detection of Human Nuclear factor-kappa B (NF-kappaB) concentration in serum, plasma, tissue homogenates and other biological fluids.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate NF-kappaB in Human serum, plasma, tissue homogenates. An antibody specific for NF-kappaB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NF-kappaB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NF-kappaB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NF-kappaB bound in the initial step. The color development is stopped and the intensity of the color is measured.