Human STK40 ELISA Kit

Alternative name
Human SINK-homologous serine/threonine-protein kinase ELISA Kit;Human sugen kinase 495 ELISA Kit;Human SgK495 ELISA Kit;Human SHIK ELISA Kit;Human serine/threonine kinase 40 ELISA Kit;Human serine/threonine-protein kinase 40 ELISA Kit;Human Ser/Thr-like kinase ELISA Kit;
Catalog
E034789
species
Human
GeneSTK40
SpecificityThis assay has high sensitivity and excellent specificity for detection of STK40. No significant cross-reactivity or interference between STK40 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between STK40 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman STK40 ELISA Kit allows for the in vitro quantitative determination of STK40 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilySignal Transduction
Product Description
specificalIntended Uses: This STK40 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human STK40. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||STK40 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-STK40 antibody and an STK40-HRP conjugate. The assay sample and buffer are incubated together with STK40-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the STK40 concentration since STK40 from samples and STK40-HRP conjugate compete for the anti-STK40 antibody binding site. Since the number of sites is limited, as more sites are occupied by STK40 from the sample, fewer sites are left to bind STK40-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The STK40 concentration in each sample is interpolated from this standard curve.
Human Serine/threonine-protein kinase 40 Protein information
Uniprot ID STK40_HUMAN
Uniprot AC Q96I44; Q9BSA3; Q9H7H6;
UniGene Hs.471768;
GeneID 83931
KEGG hsa:83931;
Human Serine/threonine-protein kinase 40 Protein SEQUENCE
SEQUENCE 435 AA; 49001 MW; EBA7A19963582BCC CRC64;
MKRRASDRGA GETSARAKAL GSGISGNNAK RAGPFILGPR LGNSPVPSIV
QCLARKDGTD DFYQLKILTL EERGDQGIES QEERQGKMLL HTEYSLLSLL
HTQDGVVHHH GLFQDRTCEI VEDTESSRMV KKMKKRICLV LDCLCAHDFS
DKTADLINLQ HYVIKEKRLS ERETVVIFYD VVRVVEALHQ KNIVHRDLKL
GNMVLNKRTH RITITNFCLG KHLVSEGDLL KDQRGSPAYI SPDVLSGRPY
RGKPSDMWAL GVVLFTMLYG QFPFYDSIPQ ELFRKIKAAE YTIPEDGRVS
ENTVCLIRKL LVLDPQQRLA AADVLEALSA IIASWQSLSS LSGPLQVVPD
IDDQMSNADS SQEAKVTEEC SQYEFENYMR QQLLLAEEKS SIHDARSWVP
KRQFGSAPPV RRLGHDAQPM TSLDTAILAQ RYLRK
UCSC uc001cak.3; human. [Q8N2I9-1];

Category

Human STK40 ELISA Kit

Human SINK-homologous serine/threonine-protein kinase ELISA Kit;Human sugen kinase 495 ELISA Kit;Human SgK495 ELISA Kit;Human SHIK ELISA Kit;Human serine/threonine kinase 40 ELISA Kit;Human serine/threonine-protein kinase 40 ELISA Kit;Human Ser/Thr-like kinase ELISA Kit;