Human HSD11B1 ELISA Kit

Alternative name
Human 11-beta-hydroxysteroid dehydrogenase 1 ELISA Kit;Human 11-DH ELISA Kit;Human 11-beta-HSD1 ELISA Kit;Human short chain dehydrogenase/reductase family 26C member 1 ELISA Kit;Human HSD11 ELISA Kit;Human HSD11L ELISA Kit;Human SDR26C1 ELISA Kit;Human CORTRD2 ELISA Kit;Human HDL ELISA Kit;Human HSD11B ELISA Kit;Human hydroxysteroid 11-beta dehydrogenase 1 ELISA Kit;Human corticosteroid 11-beta-dehydrogenase isozyme 1 ELISA Kit;
Catalog
E041686
species
Human
GeneHSD11B1
SpecificityThis assay has high sensitivity and excellent specificity for detection of 11-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD11b1). No significant cross-reactivity or interference between 11-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD11b1) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.56ng/mL.
Intended UseHuman HSD11B1 ELISA Kit allows for the in vitro quantitative determination of HSD11B1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 11-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD11b1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to 11-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD11b1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain 11-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD11b1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 11-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD11b1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Corticosteroid 11-beta-dehydrogenase isozyme 1 Protein information
Uniprot ID DHI1_HUMAN
Uniprot AC P28845; B2R9Z1; D3DT89;
UniGene Hs.195040;
GeneID 3290
KEGG hsa:3290;
Human Corticosteroid 11-beta-dehydrogenase isozyme 1 Protein SEQUENCE
SEQUENCE 292 AA; 32401 MW; 4632D0F3BBEFC474 CRC64;
MAFMKKYLLP ILGLFMAYYY YSANEEFRPE MLQGKKVIVT GASKGIGREM
AYHLAKMGAH VVVTARSKET LQKVVSHCLE LGAASAHYIA GTMEDMTFAE
QFVAQAGKLM GGLDMLILNH ITNTSLNLFH DDIHHVRKSM EVNFLSYVVL
TVAALPMLKQ SNGSIVVVSS LAGKVAYPMV AAYSASKFAL DGFFSSIRKE
YSVSRVNVSI TLCVLGLIDT ETAMKAVSGI VHMQAAPKEE CALEIIKGGA
LRQEEVYYDS SLWTTLLIRN PCRKILEFLY STSYNMDRFI NK

Human HSD11B1 ELISA Kit

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