Human HSD11B1 ELISA Kit

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  • Alternative name

    Human 11-beta-hydroxysteroid dehydrogenase 1 ELISA Kit;Human 11-DH ELISA Kit;Human 11-beta-HSD1 ELISA Kit;Human short chain dehydrogenase/reductase family 26C member 1 ELISA Kit;Human HSD11 ELISA Kit;Human HSD11L ELISA Kit;Human SDR26C1 ELISA Kit;Human CORTRD2 ELISA Kit;Human HDL ELISA Kit;Human HSD11B ELISA Kit;Human hydroxysteroid 11-beta dehydrogenase 1 ELISA Kit;Human corticosteroid 11-beta-dehydrogenase isozyme 1 ELISA Kit;

  • Catalog
    E041686
  • species
    Human
  • GeneHSD11B1
  • Standard CurveHuman HSD11B1 ELISA Kit
  • Other Species Mouse Hsd11b1 ELISA KitGuinea Pig HSD11B1 ELISA KitRabbit HSD11B1 ELISA KitRat Hsd11b1 ELISA KitSheep HSD11B1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of 11-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD11b1). No significant cross-reactivity or interference between 11-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD11b1) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.56ng/mL.
  • Intended UseHuman HSD11B1 ELISA Kit allows for the in vitro quantitative determination of HSD11B1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 11-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD11b1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to 11-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD11b1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain 11-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD11b1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 11-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD11b1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Corticosteroid 11-beta-dehydrogenase isozyme 1 Protein information
  • Uniprot ID DHI1_HUMAN
  • Uniprot AC P28845; B2R9Z1; D3DT89;
  • UniGene Hs.195040;
  • GeneID 3290
  • KEGG hsa:3290;
  • Human Corticosteroid 11-beta-dehydrogenase isozyme 1 Protein SEQUENCE
  • SEQUENCE 292 AA; 32401 MW; 4632D0F3BBEFC474 CRC64;

    MAFMKKYLLP ILGLFMAYYY YSANEEFRPE MLQGKKVIVT GASKGIGREM

    AYHLAKMGAH VVVTARSKET LQKVVSHCLE LGAASAHYIA GTMEDMTFAE

    QFVAQAGKLM GGLDMLILNH ITNTSLNLFH DDIHHVRKSM EVNFLSYVVL

    TVAALPMLKQ SNGSIVVVSS LAGKVAYPMV AAYSASKFAL DGFFSSIRKE

    YSVSRVNVSI TLCVLGLIDT ETAMKAVSGI VHMQAAPKEE CALEIIKGGA

    LRQEEVYYDS SLWTTLLIRN PCRKILEFLY STSYNMDRFI NK



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