Human IL27 ELISA Kit

Alternative name
Human IL-27 subunit alpha ELISA Kit;Human IL-27-A ELISA Kit;Human IL27-A ELISA Kit;Human interleukin-30 ELISA Kit;Human p28 ELISA Kit;Human IL27A ELISA Kit;Human IL30 ELISA Kit;Human IL-27 ELISA Kit;Human IL-27A ELISA Kit;Human IL27p28 ELISA Kit;Human interleukin 27 ELISA Kit;Human interleukin-27 subunit alpha ELISA Kit;Human IL-27 p28 subunit ELISA Kit;
Catalog
E019448
species
Human
GeneIL27
SpecificityNatural and recombinant human IL-27
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 10pg/ml
Intended UseHuman IL27 ELISA Kit allows for the in vitro quantitative determination of IL27 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: human IL-27 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-27 has been precoated onto 96-well plates. Standards(NSO,EBI3(R21-K229)+P28(F29-P243)) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-27 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-27 amount of sample captured in plate. Background: Interleukin-27 (IL-27) is a heterodimeric cytokine belonging to the IL-12 family that is composed of two subunits; Epstein-Barr virus (EBV)-induced gene 3 (EBI3) (also known as IL-27B) and IL27-p28 (known as IL-30). IL-27 is produced by antigen-presenting cells.IL-27 plays an important function in regulating the activity of B- and T-lymphocytes. IL-27, a potent inhibitor of T(H)-17 cell development, may be a useful target for treating inflammatory diseases mediated by these cells.
Human Interleukin-27 subunit alpha Protein information
Uniprot ID IL27A_HUMAN
Uniprot AC Q8NEV9; A0N0L2; Q6P676;
UniGene Hs.528111;
GeneID 246778
KEGG hsa:246778;
Human Interleukin-27 subunit alpha Protein SEQUENCE
SEQUENCE 243 AA; 27493 MW; 2FA8B8AB60B606E8 CRC64;
MGQTAGDLGW RLSLLLLPLL LVQAGVWGFP RPPGRPQLSL QELRREFTVS
LHLARKLLSE VRGQAHRFAE SHLPGVNLYL LPLGEQLPDV SLTFQAWRRL
SDPERLCFIS TTLQPFHALL GGLGTQGRWT NMERMQLWAM RLDLRDLQRH
LRFQVLAAGF NLPEEEEEEE EEEEEERKGL LPGALGSALQ GPAQVSWPQL
LSTYRLLHSL ELVLSRAVRE LLLLSKAGHS VWPLGFPTLS PQP
UCSC uc002dqc.3; human.;

Human IL27 ELISA Kit

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