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Human IL27 ELISA Kit

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  • Alternative name

    Human IL-27 subunit alpha ELISA Kit;Human IL-27-A ELISA Kit;Human IL27-A ELISA Kit;Human interleukin-30 ELISA Kit;Human p28 ELISA Kit;Human IL27A ELISA Kit;Human IL30 ELISA Kit;Human IL-27 ELISA Kit;Human IL-27A ELISA Kit;Human IL27p28 ELISA Kit;Human interleukin 27 ELISA Kit;Human interleukin-27 subunit alpha ELISA Kit;Human IL-27 p28 subunit ELISA Kit;
  • Catalog
    E019448
  • species
    Human
  • GeneIL27
  • SpecificityNatural and recombinant human IL-27
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity< 10pg/ml
  • Intended UseHuman IL27 ELISA Kit allows for the in vitro quantitative determination of IL27 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the assay: human IL-27 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-27 has been precoated onto 96-well plates. Standards(NSO,EBI3(R21-K229)+P28(F29-P243)) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-27 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-27 amount of sample captured in plate. Background: Interleukin-27 (IL-27) is a heterodimeric cytokine belonging to the IL-12 family that is composed of two subunits; Epstein-Barr virus (EBV)-induced gene 3 (EBI3) (also known as IL-27B) and IL27-p28 (known as IL-30). IL-27 is produced by antigen-presenting cells.IL-27 plays an important function in regulating the activity of B- and T-lymphocytes. IL-27, a potent inhibitor of T(H)-17 cell development, may be a useful target for treating inflammatory diseases mediated by these cells.
  • Human Interleukin-27 subunit alpha Protein information
  • Uniprot ID IL27A_HUMAN
  • Uniprot AC Q8NEV9; A0N0L2; Q6P676;
  • UniGene Hs.528111;
  • GeneID 246778
  • KEGG hsa:246778;
  • Human Interleukin-27 subunit alpha Protein SEQUENCE

  • SEQUENCE 243 AA; 27493 MW; 2FA8B8AB60B606E8 CRC64;

    MGQTAGDLGW RLSLLLLPLL LVQAGVWGFP RPPGRPQLSL QELRREFTVS

    LHLARKLLSE VRGQAHRFAE SHLPGVNLYL LPLGEQLPDV SLTFQAWRRL

    SDPERLCFIS TTLQPFHALL GGLGTQGRWT NMERMQLWAM RLDLRDLQRH

    LRFQVLAAGF NLPEEEEEEE EEEEEERKGL LPGALGSALQ GPAQVSWPQL

    LSTYRLLHSL ELVLSRAVRE LLLLSKAGHS VWPLGFPTLS PQP

  • UCSC uc002dqc.3; human.;



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