Human SEMA5A ELISA Kit

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  • Alternative name

    Human Semaphorin-F ELISA Kit;Human sema F ELISA Kit;Human SEMAF ELISA Kit;Human semF ELISA Kit;Human semaphorin 5A ELISA Kit;Human semaphorin-5A ELISA Kit;Human sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A ELISA Kit;Human semaphorin F ELISA Kit;

  • Catalog
    E034608
  • species
    Human
  • GeneSEMA5A
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Semaphorin 5A (SEMA5A). No significant cross-reactivity or interference between Semaphorin 5A (SEMA5A) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.27ng/mL.
  • Intended UseHuman SEMA5A ELISA Kit allows for the in vitro quantitative determination of SEMA5A , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Semaphorin 5A (SEMA5A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Semaphorin 5A (SEMA5A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Semaphorin 5A (SEMA5A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Semaphorin 5A (SEMA5A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Semaphorin-5A Protein information
  • Uniprot ID SEM5A_HUMAN
  • Uniprot AC Q13591; D3DTC6; O60408; Q1RLL9;
  • UniGene Hs.27621;
  • GeneID 9037
  • KEGG hsa:9037;
  • Human Semaphorin-5A Protein SEQUENCE
  • SEQUENCE 1074 AA; 120615 MW; AE073413AC974CCC CRC64;

    MKGTCVIAWL FSSLGLWRLA HPEAQGTTQC QRTEHPVISY KEIGPWLREF

    RAKNAVDFSQ LTFDPGQKEL VVGARNYLFR LQLEDLSLIQ AVEWECDEAT

    KKACYSKGKS KEECQNYIRV LLVGGDRLFT CGTNAFTPVC TNRSLSNLTE

    IHDQISGMAR CPYSPQHNST ALLTAGGELY AATAMDFPGR DPAIYRSLGI

    LPPLRTAQYN SKWLNEPNFV SSYDIGNFTY FFFRENAVEH DCGKTVFSRA

    ARVCKNDIGG RFLLEDTWTT FMKARLNCSR PGEVPFYYNE LQSTFFLPEL

    DLIYGIFTTN VNSIAASAVC VFNLSAIAQA FSGPFKYQEN SRSAWLPYPN

    PNPHFQCGTV DQGLYVNLTE RNLQDAQKFI LMHEVVQPVT TVPSFMEDNS

    RFSHVAVDVV QGREALVHII YLATDYGTIK KVRVPLNQTS SSCLLEEIEL

    FPERRREPIR SLQILHSQSV LFVGLREHVV KIPLKRCQFY RTRSTCIGAQ

    DPYCGWDVVM KKCTSLEESL SMTQWEQSIS ACPTRNLTVD GHFGVWSPWT

    PCTHTDGSAV GSCLCRTRSC DSPAPQCGGW QCEGPGMEIA NCSRNGGWTP

    WTSWSPCSTT CGIGFQVRQR SCSNPTPRHG GRVCVGQNRE ERYCNEHLLC

    PPHMFWTGWG PWERCTAQCG GGIQARRRIC ENGPDCAGCN VEYQSCNTNP

    CPELKKTTPW TPWTPVNISD NGGHYEQRFR YTCKARLADP NLLEVGRQRI

    EMRYCSSDGT SGCSTDGLSG DFLRAGRYSA HTVNGAWSAW TSWSQCSRDC

    SRGIRNRKRV CNNPEPKYGG MPCLGPSLEY QECNILPCPV DGVWSCWSPW

    TKCSATCGGG HYMRTRSCSN PAPAYGGDIC LGLHTEEALC NTQPCPESWS

    EWSDWSECEA SGVQVRARQC ILLFPMGSQC SGNTTESRPC VFDSNFIPEV

    SVARSSSVEE KRCGEFNMFH MIAVGLSSSI LGCLLTLLVY TYCQRYQQQS

    HDATVIHPVS PAPLNTSITN HINKLDKYDS VEAIKAFNKN NLILEERNKY

    FNPHLTGKTY SNAYFTDLNN YDEY




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