Human ANXA7 ELISA Kit

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  • Alternative name

    Human annexin VII ELISA Kit;Human annexin-7 ELISA Kit;Human SYNEXIN ELISA Kit;Human ANX7 ELISA Kit;Human SNX ELISA Kit;Human annexin A7 ELISA Kit;

  • Catalog
    E002431
  • species
    Human
  • GeneANXA7
  • Standard CurveHuman ANXA7 ELISA Kit
  • Other Species Mouse Anxa7 ELISA KitBovine ANXA7 ELISA KitMonkey ANXA7 ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Intended UseHuman ANXA7 ELISA Kit allows for the in vitro quantitative determination of ANXA7 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyCell Biology
  • Product Description
    specifical
    Principle of the Assay: ANXA7 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ANXA7 antibody and an ANXA7-HRP conjugate. The assay sample and buffer are incubated together with ANXA7-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ANXA7 concentration since ANXA7 from samples and ANXA7-HRP conjugate compete for the anti-ANXA7 antibody binding site. Since the number of sites is limited, as more sites are occupied by ANXA7 from the sample, fewer sites are left to bind ANXA7-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ANXA7 concentration in each sample is interpolated from this standard curve.
  • Human Annexin A7 Protein information
  • Uniprot ID ANXA7_HUMAN
  • Uniprot AC P20073; Q5F2H3; Q5T0M6; Q5T0M7;
  • UniGene Hs.631827;
  • GeneID 310
  • KEGG hsa:310;
  • Human Annexin A7 Protein SEQUENCE
  • SEQUENCE 488 AA; 52739 MW; BFC688479D8CC2A0 CRC64;

    MSYPGYPPTG YPPFPGYPPA GQESSFPPSG QYPYPSGFPP MGGGAYPQVP

    SSGYPGAGGY PAPGGYPAPG GYPGAPQPGG APSYPGVPPG QGFGVPPGGA

    GFSGYPQPPS QSYGGGPAQV PLPGGFPGGQ MPSQYPGGQP TYPSQINTDS

    FSSYPVFSPV SLDYSSEPAT VTQVTQGTIR PAANFDAIRD AEILRKAMKG

    FGTDEQAIVD VVANRSNDQR QKIKAAFKTS YGKDLIKDLK SELSGNMEEL

    ILALFMPPTY YDAWSLRKAM QGAGTQERVL IEILCTRTNQ EIREIVRCYQ

    SEFGRDLEKD IRSDTSGHFE RLLVSMCQGN RDENQSINHQ MAQEDAQRLY

    QAGEGRLGTD ESCFNMILAT RSFPQLRATM EAYSRMANRD LLSSVSREFS

    GYVESGLKTI LQCALNRPAF FAERLYYAMK GAGTDDSTLV RIVVTRSEID

    LVQIKQMFAQ MYQKTLGTMI AGDTSGDYRR LLLAIVGQ

  • UCSC uc001jtz.3; human. [P20073-1];


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