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Human SFRP4 ELISA Kit

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  • Alternative name

    Human sFRP-4 ELISA Kit;Human frizzled protein, human endometrium ELISA Kit;Human FRPHE ELISA Kit;Human FRP-4 ELISA Kit;Human PYL ELISA Kit;Human secreted frizzled related protein 4 ELISA Kit;Human secreted frizzled-related protein 4 ELISA Kit;Human secreted frizzled-related protein 4; secreted frizzled-related protein 4 ELISA Kit;
  • Catalog
    E034428
  • species
    Human
  • GeneSFRP4
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Secreted Frizzled Related Protein 4 (SFRP4). No significant cross-reactivity or interference between Secreted Frizzled Related Protein 4 (SFRP4) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 6.1pg/mL.
  • Intended UseHuman SFRP4 ELISA Kit allows for the in vitro quantitative determination of SFRP4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Secreted Frizzled Related Protein 4 (SFRP4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Secreted Frizzled Related Protein 4 (SFRP4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Secreted Frizzled Related Protein 4 (SFRP4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Secreted Frizzled Related Protein 4 (SFRP4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Secreted frizzled-related protein 4 Protein information
  • Uniprot ID SFRP4_HUMAN
  • Uniprot AC Q6FHJ7; B4DYC1; O14877; Q05BG7; Q1ZYW2; Q4G124; Q6FHM0; Q6PD64;
  • UniGene Hs.658169;
  • GeneID 6424
  • KEGG hsa:6424;
  • Human Secreted frizzled-related protein 4 Protein SEQUENCE

  • SEQUENCE 346 AA; 39827 MW; 75D04089E446CE80 CRC64;

    MFLSILVALC LWLHLALGVR GAPCEAVRIP MCRHMPWNIT RMPNHLHHST

    QENAILAIEQ YEELVDVNCS AVLRFFLCAM YAPICTLEFL HDPIKPCKSV

    CQRARDDCEP LMKMYNHSWP ESLACDELPV YDRGVCISPE AIVTDLPEDV

    KWIDITPDMM VQERPLDVDC KRLSPDRCKC KKVKPTLATY LSKNYSYVIH

    AKIKAVQRSG CNEVTTVVDV KEIFKSSSPI PRTQVPLITN SSCQCPHILP

    HQDVLIMCYE WRSRMMLLEN CLVEKWRDQL SKRSIQWEER LQEQRRTVQD

    KKKTAGRTSR SNPPKPKGKP PAPKPASPKK NIKTRSAQKR TNPKRV

  • UCSC uc003tfo.5; human.;



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