Human CLCF1 ELISA Kit

Alternative name
Human B-cell-stimulating factor 3 ELISA Kit;Human BSF-3 ELISA Kit;Human novel neurotrophin-1 ELISA Kit;Human NNT-1 ELISA Kit;Human BSF3 ELISA Kit;Human CLC ELISA Kit;Human NNT1 ELISA Kit;Human CISS2 ELISA Kit;Human NR6 ELISA Kit;Human cardiotrophin-like cytokine factor 1 ELISA Kit;Human B-cell stimulatory factor 3 ELISA Kit;Human CRLF1 associated cytokine-like factor 1 ELISA Kit;Human neurotrophin-1/B-cell stimulating factor-3 ELISA Kit;
Catalog
E006713
species
Human
GeneCLCF1
Standard Curve
Other Species Mouse Clcf1 ELISA Kit
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 pg/mL.
Intended UseHuman CLCF1 ELISA Kit allows for the in vitro quantitative determination of CLCF1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyCardiovascular
Product Description
specificalFor samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate INTENDED USE This CLCF1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CLCF1. This ELISA kit for research use only, not for therapeutic or diagnostic applications! PRINCIPLE OF THE ASSAY CLCF1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for CLCF1. Standards or samples are then added to the microtiter plate wells and CLCF1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of CLCF1 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for CLCF1 are added to each well to ?sandwich? the CLCF1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain CLCF1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CLCF1 concentration in each sample is interpolated from this standard curve.
Human Cardiotrophin-like cytokine factor 1 Protein information
Uniprot ID CLCF1_HUMAN
Uniprot AC Q9UBD9; B4DNT4; Q6NZA4;
UniGene Hs.502977;
GeneID 23529
KEGG hsa:23529;
Human Cardiotrophin-like cytokine factor 1 Protein SEQUENCE
SEQUENCE 225 AA; 25176 MW; E2DD4B6280833B55 CRC64;
MDLRAGDSWG MLACLCTVLW HLPAVPALNR TGDPGPGPSI QKTYDLTRYL
EHQLRSLAGT YLNYLGPPFN EPDFNPPRLG AETLPRATVD LEVWRSLNDK
LRLTQNYEAY SHLLCYLRGL NRQAATAELR RSLAHFCTSL QGLLGSIAGV
MAALGYPLPQ PLPGTEPTWT PGPAHSDFLQ KMDDFWLLKE LQTWLWRSAK
DFNRLKKKMQ PPAAAVTLHL GAHGF
UCSC uc001okq.4; human. [Q9UBD9-1];

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Human CLCF1 ELISA Kit