Human DIO2 ELISA Kit

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  • Alternative name

    Human 5DII ELISA Kit;Human DIOII ELISA Kit;Human type 2 DI ELISA Kit;Human type-II 5'-deiodinase ELISA Kit;Human ITDI2 ELISA Kit;Human TXDI2 ELISA Kit;Human D2 ELISA Kit;Human SelY ELISA Kit;Human iodothyronine deiodinase 2 ELISA Kit;Human type II iodothyronine deiodinase ELISA Kit;Human deiodinase, iodothyronine type II ELISA Kit;Human deiodinase-2 ELISA Kit;Human deiodonase-2 ELISA Kit;Human thyroxine deiodinase, type II ELISA Kit;Human type 2 iodothyronine deiodinase ELISA Kit;Human type-II 5'deiodinase ELISA Kit;

  • Catalog
    E011576
  • species
    Human
  • GeneDIO2
  • Standard CurveHuman DIO2 ELISA Kit
  • Other Species Mouse Dio2 ELISA KitBovine DIO2 ELISA KitChicken DIO2 ELISA KitPorcine DIO2 ELISA KitRat Dio2 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Deiodinase, Iodothyronine, Type II (DIO2). No significant cross-reactivity or interference between Deiodinase, Iodothyronine, Type II (DIO2) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.055ng/mL.
  • Intended UseHuman DIO2 ELISA Kit allows for the in vitro quantitative determination of DIO2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Deiodinase, Iodothyronine, Type II (DIO2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Deiodinase, Iodothyronine, Type II (DIO2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Deiodinase, Iodothyronine, Type II (DIO2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Deiodinase, Iodothyronine, Type II (DIO2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Type II iodothyronine deiodinase Protein information
  • Uniprot ID IOD2_HUMAN
  • Uniprot AC Q92813; B9EGK0; G3V315; Q6B0A3; Q9HCP8; Q9P1W4; Q9UDZ1;
  • UniGene Hs.202354; Hs.708526; Hs.732170;
  • GeneID 1734
  • KEGG hsa:1734;
  • Human Type II iodothyronine deiodinase Protein SEQUENCE
  • SEQUENCE 273 AA; 30552 MW; 88D68B9AA6CB2A42 CRC64;

    MGILSVDLLI TLQILPVFFS NCLFLALYDS VILLKHVVLL LSRSKSTRGE

    WRRMLTSEGL RCVWKSFLLD AYKQVKLGED APNSSVVHVS STEGGDNSGN

    GTQEKIAEGA TCHLLDFASP ERPLVVNFGS ATUPPFTSQL PAFRKLVEEF

    SSVADFLLVY IDEAHPSDGW AIPGDSSLSF EVKKHQNQED RCAAAQQLLE

    RFSLPPQCRV VADRMDNNAN IAYGVAFERV CIVQRQKIAY LGGKGPFSYN

    LQEVRHWLEK NFSKRUKKTR LAG

  • UCSC uc010asy.4; human. [Q92813-1];



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