Mouse Vip ELISA Kit

Alternative name
Mouse Peptide histidine isoleucinamide 27 ELISA Kit;Mouse VIP ELISA Kit;Mouse vasoactive intestinal polypeptide ELISA Kit;Mouse VIP peptides ELISA Kit;Mouse PHI, peptide histidine isoleucine ELISA Kit;
Catalog
E072394
species
Mouse
GeneVip
SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse VIP. No significant cross-reactivity or interference between Mouse VIP and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.47 pg/ml
Intended UseMouse Vip ELISA Kit allows for the in vitro quantitative determination of Vip , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalIntroduction: Vasoactive intestinal peptide (VIP, also polypeptide) is a peptide hormone containing 28 amino acid residues. VIP is very widely distributed in the peripheral and central nervous systems, and probably should not be considered a true GI hormone. A huge number of biological effects have been attributed to VIP. With respect to the digestive system, VIP seems to induce smooth muscle relaxation (lower esophageal sphincter, stomach, gallbladder), stimulate secretion of water into pancreatic juice and bile, and cause inhibition of gastric acid secretion and absorption from the intestinal lumen. Certain tumors arising from the pancreatic islets or nervous tissue (called VIPomas) secrete excessive quantities of VIP, and are associated with chronic, watery diarrhea. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to VIP. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for VIP and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain VIP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of VIP in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Mouse Vasoactive intestinal peptide Protein information
Uniprot ID VIP_MOUSE
Uniprot AC P32648; Q9D2Z7; Q9QUN1;
UniGene Mm.98916;
GeneID 22353
KEGG mmu:22353;
Mouse Vasoactive intestinal peptide Protein SEQUENCE
SEQUENCE 170 AA; 19049 MW; 0164C831F8F5C73D CRC64;
MEARSKPQFL AFLILFSVLF SQSLAWPLFG PPSVVRLDDR MPFEGAGDPD
QVSLKADSDI LQNPLAENGT PYYDVSRNAR HADGVFTSDY SRLLGQISAK
KYLESLIGKR ISSSISEDPV PIKRHSDAVF TDNYTRLRKQ MAVKKYLNSI
LNGKRSSEGD SADFLEELEK
UCSC uc007egk.1; mouse.;

Mouse Vip ELISA Kit

Mouse Peptide histidine isoleucinamide 27 ELISA Kit;Mouse VIP ELISA Kit;Mouse vasoactive intestinal polypeptide ELISA Kit;Mouse VIP peptides ELISA Kit;Mouse PHI, peptide histidine isoleucine ELISA Kit;

Contact Us