Human CPN1 ELISA Kit

Alternative name
Human CPN ELISA Kit;Human anaphylatoxin inactivator ELISA Kit;Human arginine carboxypeptidase ELISA Kit;Human Carboxypeptidase N polypeptide 1 ELISA Kit;Human carboxypeptidase N small subunit ELISA Kit;Human kininase-1 ELISA Kit;Human lysine carboxypeptidase ELISA Kit;Human plasma carboxypeptidase B ELISA Kit;Human serum carboxypeptidase N ELISA Kit;Human SCPN ELISA Kit;Human ACBP ELISA Kit;Human carboxypeptidase N subunit 1 ELISA Kit;Human carboxypeptidase N catalytic chain ELISA Kit;Human carboxypeptidase K ELISA Kit;Human carboxypeptidase N catalytic subunit ELISA Kit;Human carboxypeptidase N polypeptide 1 50 kD ELISA Kit;Human carboxypeptidase N, polypeptide 1 ELISA Kit;Human kininase I ELISA Kit;
Catalog
E006605
species
Human
GeneCPN1
Standard Curve
Other Species Mouse Cpn1 ELISA Kit Bovine CPN1 ELISA Kit Rat Cpn1 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of CPN1. No significant cross-reactivity or interference between CPN1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CPN1 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman CPN1 ELISA Kit allows for the in vitro quantitative determination of CPN1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalIntended Uses: This CPN1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CPN1. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||CPN1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-CPN1 antibody and an CPN1-HRP conjugate. The assay sample and buffer are incubated together with CPN1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CPN1 concentration since CPN1 from samples and CPN1-HRP conjugate compete for the anti-CPN1 antibody binding site. Since the number of sites is limited, as more sites are occupied by CPN1 from the sample, fewer sites are left to bind CPN1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CPN1 concentration in each sample is interpolated from this standard curve.
Human Carboxypeptidase N catalytic chain Protein information
Uniprot ID CBPN_HUMAN
Uniprot AC P15169; B1AP59;
UniGene Hs.2246;
GeneID 1369
KEGG hsa:1369;
Human Carboxypeptidase N catalytic chain Protein SEQUENCE
SEQUENCE 458 AA; 52286 MW; 9BEF3E459E291E39 CRC64;
MSDLLSVFLH LLLLFKLVAP VTFRHHRYDD LVRTLYKVQN ECPGITRVYS
IGRSVEGRHL YVLEFSDHPG IHEPLEPEVK YVGNMHGNEA LGRELMLQLS
EFLCEEFRNR NQRIVQLIQD TRIHILPSMN PDGYEVAAAQ GPNKPGYLVG
RNNANGVDLN RNFPDLNTYI YYNEKYGGPN HHLPLPDNWK SQVEPETRAV
IRWMHSFNFV LSANLHGGAV VANYPYDKSF EHRVRGVRRT ASTPTPDDKL
FQKLAKVYSY AHGWMFQGWN CGDYFPDGIT NGASWYSLSK GMQDFNYLHT
NCFEITLELS CDKFPPEEEL QREWLGNREA LIQFLEQVHQ GIKGMVLDEN
YNNLANAVIS VSGINHDVTS GDHGDYFRLL LPGIYTVSAT APGYDPETVT
VTVGPAEPTL VNFHLKRSIP QVSPVRRAPS RRHGVRAKVQ PQARKKEMEM
RQLQRGPA
UCSC uc001kql.3; human.;

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Human CPN1 ELISA Kit