-
Alternative name
Human Calgranulin-B ELISA Kit;Human calprotectin L1H subunit ELISA Kit;Human leukocyte L1 complex heavy chain ELISA Kit;Human migration inhibitory factor-related protein 14 ELISA Kit;Human MRP-14 ELISA Kit;Human P14 ELISA Kit;Human S100 calcium-binding protein A9 ELISA Kit;Human CAGB ELISA Kit;Human CFAG ELISA Kit;Human MRP14 ELISA Kit;Human 60B8AG ELISA Kit;Human CGLB ELISA Kit;Human L1AG ELISA Kit;Human LIAG ELISA Kit;Human MAC387 ELISA Kit;Human MIF ELISA Kit;Human NIF ELISA Kit;Human S100 calcium binding protein A9 ELISA Kit;Human protein S100-A9 ELISA Kit;Human calgranulin B ELISA Kit;
-
Catalog
E034112
-
species
Human
-
GeneS100A9
-
SpecificityThis assay has high sensitivity and excellent specificity for detection of S100A9. No significant cross-reactivity or interference between S100A9 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between S100A9 and all the analogues, therefore, cross reaction may still exist in some cases.
-
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
-
Sensitivity0.1 ng/mL.
-
Intended UseHuman S100A9 ELISA Kit allows for the in vitro quantitative determination of S100A9 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
-
StorageStore the whole ELISA kit at 4℃
-
Product Categories/FamilySignal Transduction
-
Product Description
specificalIntended Uses: This S100A9 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human S100A9. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Principle of the Assay||S100A9 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-S100A9 antibody and an S100A9-HRP conjugate. The assay sample and buffer are incubated together with S100A9-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the S100A9 concentration since S100A9 from samples and S100A9-HRP conjugate compete for the anti-S100A9 antibody binding site. Since the number of sites is limited, as more sites are occupied by S100A9 from the sample, fewer sites are left to bind S100A9-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The S100A9 concentration in each sample is interpolated from this standard curve.
-
Human Protein S100-A9 Protein information
-
Uniprot ID
S10A9_HUMAN
-
Uniprot AC
P06702; D3DV36; Q6FGA1; Q9NYM0; Q9UCJ1;
-
UniGene
Hs.112405;
-
GeneID
6280
-
KEGG
hsa:6280;
-
Human Protein S100-A9 Protein SEQUENCE
-
SEQUENCE 114 AA; 13242 MW; C3BE19729E14C078 CRC64;
MTCKMSQLER NIETIINTFH QYSVKLGHPD TLNQGEFKEL VRKDLQNFLK
KENKNEKVIE
HIMEDLDTNA DKQLSFEEFI MLMARLTWAS HEKMHEGDEG
PGHHHKPGLG EGTP
-
UCSC
uc001fbq.3; human.;
-