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Alternative name
Testican-1 ELISA KIT; Protein SPOCKSPOCK1 ELISA KIT; SPOCK ELISA KIT; TIC1 ELISA KIT; TICN1 ELISA KIT
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Catalog
E037749
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species
Human
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GeneTIC1
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SpecificityThis assay has high sensitivity and excellent specificity for detection of TIC1. No significant cross-reactivity or interference between TIC1 and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityThe minimum detectable dose of TIC1 is typically less than 0.067ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two
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Intended UseHuman TIC1 ELISA Kit allows for the in vitro quantitative determination of TIC1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to TIC1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to TIC1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TIC1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of TIC1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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