Human E2F3 ELISA Kit

Alternative name
Human E2F-3 ELISA Kit;Human KIAA0075 ELISA Kit;Human E2F transcription factor 3 ELISA Kit;Human transcription factor E2F3 ELISA Kit;
Catalog
E012629
species
Human
GeneE2F3
Standard Curve
Other Species Mouse E2f3 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of E2F Transcription Factor 3 (E2F3). No significant cross-reactivity or interference between E2F Transcription Factor 3 (E2F3) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.072ng/mL.
Intended UseHuman E2F3 ELISA Kit allows for the in vitro quantitative determination of E2F3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to E2F Transcription Factor 3 (E2F3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to E2F Transcription Factor 3 (E2F3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain E2F Transcription Factor 3 (E2F3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of E2F Transcription Factor 3 (E2F3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Transcription factor E2F3 Protein information
Uniprot ID E2F3_HUMAN
Uniprot AC O00716; Q15000; Q68DT0; Q9BZ44;
UniGene Hs.269408; Hs.703174;
GeneID 1871
KEGG hsa:1871;
Human Transcription factor E2F3 Protein SEQUENCE
SEQUENCE 465 AA; 49162 MW; 4641565842CA99EC CRC64;
MRKGIQPALE QYLVTAGGGE GAAVVAAAAA ASMDKRALLA SPGFAAAAAA
AAAPGAYIQI LTTNTSTTSC SSSLQSGAVA AGPLLPSAPG AEQTAGSLLY
TTPHGPSSRA GLLQQPPALG RGGSGGGGGP PAKRRLELGE SGHQYLSDGL
KTPKGKGRAA LRSPDSPKTP KSPSEKTRYD TSLGLLTKKF IQLLSQSPDG
VLDLNKAAEV LKVQKRRIYD ITNVLEGIHL IKKKSKNNVQ WMGCSLSEDG
GMLAQCQGLS KEVTELSQEE KKLDELIQSC TLDLKLLTED SENQRLAYVT
YQDIRKISGL KDQTVIVVKA PPETRLEVPD SIESLQIHLA STQGPIEVYL
CPEETETHSP MKTNNQDHNG NIPKPASKDL ASTNSGHSDC SVSMGNLSPL
ASPANLLQQT EDQIPSNLEG PFVNLLPPLL QEDYLLSLGE EEGISDLFDA
YDLEKLPLVE DFMCS
UCSC uc003nda.3; human. [O00716-1];

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Category

Human E2F3 ELISA Kit

Human E2F-3 ELISA Kit;Human KIAA0075 ELISA Kit;Human E2F transcription factor 3 ELISA Kit;Human transcription factor E2F3 ELISA Kit;