Human SERPINA12 ELISA Kit

Alternative name
Human OL-64 ELISA Kit;Human visceral adipose tissue-derived serine protease inhibitor ELISA Kit;Human vaspin ELISA Kit;Human visceral adipose-specific SERPIN ELISA Kit;Human serpin family A member 12 ELISA Kit;Human serpin A12 ELISA Kit;Human serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 12 ELISA Kit;Human serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 12 ELISA Kit;
Catalog
E034883
species
Human
GeneSERPINA12
SpecificityThis assay has high sensitivity and excellent specificity for detection of Human SERPINA12. No significant cross-reactivity or interference between Human SERPINA12 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.78 pg/ml
Intended UseHuman SERPINA12 ELISA Kit allows for the in vitro quantitative determination of SERPINA12 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalIntroduction: Vaspin (Visceral adipose-specific SERPIN), a newly identified adipokine, which is a member of serine protease inhibitor family. Vaspin is also a unique insulin sensitizing adipocytokine in obesity. A recent publication indicates that induction of human vaspin mRNA expression in adipose tissue is regulated in a fat depot-specific manner and couil be associated with parameters of obesity, insulin resistance, and glucose metabolism. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to vaspin. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for vaspin and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain vaspin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of vaspin in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Serpin A12 Protein information
Uniprot ID SPA12_HUMAN
Uniprot AC Q8IW75;
UniGene Hs.99476;
GeneID 145264
KEGG hsa:145264;
Human Serpin A12 Protein SEQUENCE
SEQUENCE 414 AA; 47175 MW; 5C70F1AB5935661C CRC64;
MNPTLGLAIF LAVLLTVKGL LKPSFSPRNY KALSEVQGWK QRMAAKELAR
QNMDLGFKLL KKLAFYNPGR NIFLSPLSIS TAFSMLCLGA QDSTLDEIKQ
GFNFRKMPEK DLHEGFHYII HELTQKTQDL KLSIGNTLFI DQRLQPQRKF
LEDAKNFYSA ETILTNFQNL EMAQKQINDF ISQKTHGKIN NLIENIDPGT
VMLLANYIFF RARWKHEFDP NVTKEEDFFL EKNSSVKVPM MFRSGIYQVG
YDDKLSCTIL EIPYQKNITA IFILPDEGKL KHLEKGLQVD TFSRWKTLLS
RRVVDVSVPR LHMTGTFDLK KTLSYIGVSK IFEEHGDLTK IAPHRSLKVG
EAVHKAELKM DERGTEGAAG TGAQTLPMET PLVVKIDKPY LLLIYSEKIP
SVLFLGKIVN PIGK
UCSC uc001ydj.3; human.;

Category

Human SERPINA12 ELISA Kit