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Alternative name
Human Calgranulin-A ELISA Kit;Human calprotectin L1L subunit ELISA Kit;Human cystic fibrosis antigen ELISA Kit;Human CFAG ELISA Kit;Human leukocyte L1 complex light chain ELISA Kit;Human migration inhibitory factor-related protein 8 ELISA Kit;Human MRP-8 ELISA Kit;Human P8 ELISA Kit;Human S100 calcium-binding protein A8 ELISA Kit;Human urinary stone protein band A ELISA Kit;Human CAGA ELISA Kit;Human MRP8 ELISA Kit;Human 60B8AG ELISA Kit;Human CGLA ELISA Kit;Human CP-10 ELISA Kit;Human L1Ag ELISA Kit;Human MA387 ELISA Kit;Human MIF ELISA Kit;Human NIF ELISA Kit;Human S100 calcium binding protein A8 ELISA Kit;Human protein S100-A8 ELISA Kit;Human calgranulin A ELISA Kit;
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Catalog
E034108
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species
Human
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GeneS100A8
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity0.1 ng/mL.
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Intended UseHuman S100A8 ELISA Kit allows for the in vitro quantitative determination of S100A8 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageStore the whole ELISA kit at 4℃
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Product Categories/FamilySignal Transduction
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Product Description
specificalPrinciple of the Assay: S100A8 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for S100A8. Standards or samples are then added to the microtiter plate wells and S100A8 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of S100A8 present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for S100A8 are added to each well to "sandwich" the S100A8 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain S100A8 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The S100A8 concentration in each sample is interpolated from this standard curve.
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Human Protein S100-A8, N-terminally processed Protein information
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Uniprot ID
S10A8_HUMAN
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Uniprot AC
P05109; A8K5L3; D3DV37; Q5SY70; Q9UC84; Q9UC92; Q9UCJ0; Q9UCM6;
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UniGene
Hs.416073;
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GeneID
6279
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KEGG
hsa:6279;
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Human Protein S100-A8, N-terminally processed Protein SEQUENCE
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SEQUENCE 93 AA; 10835 MW; 78F589140B9CE166 CRC64;
MLTELEKALN SIIDVYHKYS LIKGNFHAVY RDDLKKLLET ECPQYIRKKG
ADVWFKELDI
NTDGAVNFQE FLILVIKMGV AAHKKSHEES HKE
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UCSC
uc001fbs.3; human.;
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