Human PNOC ELISA Kit

Alternative name
Human orphanin FQ ELISA Kit;Human PPNOC ELISA Kit;Human OFQ ELISA Kit;Human N/OFQ ELISA Kit;Human NOP ELISA Kit;Human ppN/OFQ ELISA Kit;Human prepronociceptin ELISA Kit;Human nociceptin ELISA Kit;Human nocistatin ELISA Kit;Human pre-pro-N/OFQ ELISA Kit;Human pronociceptin ELISA Kit;
Catalog
E030577
species
Human
GenePNOC
SpecificityThis assay has high sensitivity and excellent specificity for detection of Human PNOC. No significant cross-reactivity or interference between Human PNOC and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.17 pg/ml
Intended UseHuman PNOC ELISA Kit allows for the in vitro quantitative determination of PNOC , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to OFQ/N. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for OFQ/N and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain OFQ/N, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of OFQ/N in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Orphanin FQ2 Protein information
Uniprot ID PNOC_HUMAN
Uniprot AC Q13519; B7Z749; Q6FH16;
UniGene Hs.88218;
GeneID 5368
KEGG hsa:5368;
Human Orphanin FQ2 Protein SEQUENCE
SEQUENCE 176 AA; 20295 MW; 8FC8DB4BB094CE6A CRC64;
MKVLLCDLLL LSLFSSVFSS CQRDCLTCQE KLHPALDSFD LEVCILECEE
KVFPSPLWTP CTKVMARSSW QLSPAAPEHV AAALYQPRAS EMQHLRRMPR
VRSLFQEQEE PEPGMEEAGE MEQKQLQKRF GGFTGARKSA RKLANQKRFS
EFMRQYLVLS MQSSQRRRTL HQNGNV
UCSC uc003xgp.5; human. [Q13519-1];

Human PNOC ELISA Kit

Human orphanin FQ ELISA Kit;Human PPNOC ELISA Kit;Human OFQ ELISA Kit;Human N/OFQ ELISA Kit;Human NOP ELISA Kit;Human ppN/OFQ ELISA Kit;Human prepronociceptin ELISA Kit;Human nociceptin ELISA Kit;Human nocistatin ELISA Kit;Human pre-pro-N/OFQ ELISA Kit;Human pronociceptin ELISA Kit;

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