Human APOD ELISA Kit

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  • Alternative name

    Human apo-D ELISA Kit;Human ApoD ELISA Kit;Human apolipoprotein D ELISA Kit;

  • Catalog
    E003338
  • species
    Human
  • GeneAPOD
  • Standard CurveHuman APOD ELISA Kit
  • Other Species Mouse Apod ELISA KitBovine APOD ELISA KitGuinea Pig APOD ELISA KitMonkey APOD ELISA KitRabbit APOD ELISA KitRat Apod ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of ApoD. No significant cross-reactivity or interference between ApoD and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ApoD and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 pg/mL.
  • Intended UseHuman APOD ELISA Kit allows for the in vitro quantitative determination of APOD , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyNeurobiology
  • Product Description
    specifical
    Principle of the assay: ApoD ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ApoD antibody and an ApoD-HRP conjugate. The assay sample and buffer are incubated together with ApoD-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ApoD concentration since ApoD from samples and ApoD-HRP conjugate compete for the anti-ApoD antibody binding site. Since the number of sites is limited, as more sites are occupied by ApoD from the sample, fewer sites are left to bind ApoD-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ApoD concentration in each sample is interpolated from this standard curve.
  • Human Apolipoprotein D Protein information
  • Uniprot ID APOD_HUMAN
  • Uniprot AC P05090; B2R579; D3DNW6; Q6IBG6;
  • UniGene Hs.522555;
  • GeneID 347
  • KEGG hsa:347;
  • Human Apolipoprotein D Protein SEQUENCE
  • SEQUENCE 189 AA; 21276 MW; 0EAA6DE03D5E71A8 CRC64;

    MVMLLLLLSA LAGLFGAAEG QAFHLGKCPN PPVQENFDVN KYLGRWYEIE

    KIPTTFENGR CIQANYSLME NGKIKVLNQE LRADGTVNQI EGEATPVNLT

    EPAKLEVKFS WFMPSAPYWI LATDYENYAL VYSCTCIIQL FHVDFAWILA

    RNPNLPPETV DSLKNILTSN NIDVKKMTVT DQVNCPKLS

  • UCSC uc003fur.2; human.;


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