Human APOD ELISA Kit

Alternative name
Human apo-D ELISA Kit;Human ApoD ELISA Kit;Human apolipoprotein D ELISA Kit;
Catalog
E003338
species
Human
GeneAPOD
Standard Curve
Other Species Mouse Apod ELISA Kit Bovine APOD ELISA Kit Guinea Pig APOD ELISA Kit Monkey APOD ELISA Kit Rabbit APOD ELISA Kit Rat Apod ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of ApoD. No significant cross-reactivity or interference between ApoD and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ApoD and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 pg/mL.
Intended UseHuman APOD ELISA Kit allows for the in vitro quantitative determination of APOD , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyNeurobiology
Product Description
specificalPrinciple of the assay: ApoD ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ApoD antibody and an ApoD-HRP conjugate. The assay sample and buffer are incubated together with ApoD-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ApoD concentration since ApoD from samples and ApoD-HRP conjugate compete for the anti-ApoD antibody binding site. Since the number of sites is limited, as more sites are occupied by ApoD from the sample, fewer sites are left to bind ApoD-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ApoD concentration in each sample is interpolated from this standard curve.
Human Apolipoprotein D Protein information
Uniprot ID APOD_HUMAN
Uniprot AC P05090; B2R579; D3DNW6; Q6IBG6;
UniGene Hs.522555;
GeneID 347
KEGG hsa:347;
Human Apolipoprotein D Protein SEQUENCE
SEQUENCE 189 AA; 21276 MW; 0EAA6DE03D5E71A8 CRC64;
MVMLLLLLSA LAGLFGAAEG QAFHLGKCPN PPVQENFDVN KYLGRWYEIE
KIPTTFENGR CIQANYSLME NGKIKVLNQE LRADGTVNQI EGEATPVNLT
EPAKLEVKFS WFMPSAPYWI LATDYENYAL VYSCTCIIQL FHVDFAWILA
RNPNLPPETV DSLKNILTSN NIDVKKMTVT DQVNCPKLS
UCSC uc003fur.2; human.;

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Human APOD ELISA Kit

Human apo-D ELISA Kit;Human ApoD ELISA Kit;Human apolipoprotein D ELISA Kit;