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  • Alternative name

    Caspase recruitment domain-containing protein 8 ELISA KIT; Apoptotic protein NDPP1 ELISA KIT; CARD-inhibitor of NF-kappa-B-activating ligand ELISA KIT; CARDINAL ELISA KIT; DACAR ELISA KIT; Tumor up-regulated CARD-containing antagonist of CASP9 ELISA KIT; TUCANCARD8 ELISA KIT; KIAA0955 ELISA KIT; NDPP1 ELISA KIT; CARDINAL ELISA KIT; TUCAN ELISA KIT

  • Catalog
  • species
  • GeneCARD8
  • Standard CurveHuman CARD8 ELISA Kit
  • Other Species Mouse CARD8 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of CARD8. No significant cross-reactivity or interference between CARD8 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CARD8 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman CARD8 ELISA Kit allows for the in vitro quantitative determination of CARD8 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilyCell Biology
  • Product Description
    Intended Uses: This CARD8 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CARD8. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||CARD8 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CARD8 antibody and an CARD8-HRP conjugate. The assay sample and buffer are incubated together with CARD8-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CARD8 concentration since CARD8 from samples and CARD8-HRP conjugate compete for the anti-CARD8 antibody binding site. Since the number of sites is limited, as more sites are occupied by CARD8 from the sample, fewer sites are left to bind CARD8-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CARD8 concentration in each sample is interpolated from this standard curve.

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