Human RARA ELISA Kit

Alternative name
Human RAR-alpha ELISA Kit;Human nuclear receptor subfamily 1 group B member 1 ELISA Kit;Human NR1B1 ELISA Kit;Human RAR ELISA Kit;Human retinoic acid receptor alpha ELISA Kit;Human nucleophosmin-retinoic acid receptor alpha fusion protein NPM-RAR long form ELISA Kit;Human retinoic acid nuclear receptor alpha variant 1 ELISA Kit;Human retinoic acid nuclear receptor alpha variant 2 ELISA Kit;Human retinoic acid receptor, alpha polypeptide ELISA Kit;
Catalog
E033353
species
Human
GeneRARA
SpecificityThis assay has high sensitivity and excellent specificity for detection of RARa. No significant cross-reactivity or interference between RARa and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human RARa is typically less than 0.058ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by addi
Intended UseHuman RARA ELISA Kit allows for the in vitro quantitative determination of RARA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to RARa. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to RARa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain RARa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of RARa in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Retinoic acid receptor alpha Protein information
Uniprot ID RARA_HUMAN
Uniprot AC P10276; B8Y636; P78456; Q13440; Q13441; Q96S41; Q9NQS0;
UniGene Hs.654583;
GeneID 5914
KEGG hsa:5914;
Human Retinoic acid receptor alpha Protein SEQUENCE
SEQUENCE 462 AA; 50771 MW; E8D1CF9A1E57CB99 CRC64;
MASNSSSCPT PGGGHLNGYP VPPYAFFFPP MLGGLSPPGA LTTLQHQLPV
SGYSTPSPAT IETQSSSSEE IVPSPPSPPP LPRIYKPCFV CQDKSSGYHY
GVSACEGCKG FFRRSIQKNM VYTCHRDKNC IINKVTRNRC QYCRLQKCFE
VGMSKESVRN DRNKKKKEVP KPECSESYTL TPEVGELIEK VRKAHQETFP
ALCQLGKYTT NNSSEQRVSL DIDLWDKFSE LSTKCIIKTV EFAKQLPGFT
TLTIADQITL LKAACLDILI LRICTRYTPE QDTMTFSDGL TLNRTQMHNA
GFGPLTDLVF AFANQLLPLE MDDAETGLLS AICLICGDRQ DLEQPDRVDM
LQEPLLEALK VYVRKRRPSR PHMFPKMLMK ITDLRSISAK GAERVITLKM
EIPGSMPPLI QEMLENSEGL DTLSGQPGGG GRDGGGLAPP PGSCSPSLSP
SSNRSSPATH SP
UCSC uc002hun.3; human. [P10276-1];

Category

Human RARA ELISA Kit