Human ACAT2 ELISA Kit

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  • Alternative name

    Human acetyl-CoA transferase-like protein ELISA Kit;Human cytosolic acetoacetyl-CoA thiolase ELISA Kit;Human ACTL ELISA Kit;Human acetyl-CoA acetyltransferase 2 ELISA Kit;Human acetyl-CoA acetyltransferase, cytosolic ELISA Kit;Human acetoacetyl Coenzyme A thiolase ELISA Kit;

  • Catalog
    E000227
  • species
    Human
  • GeneACAT2
  • Standard CurveHuman ACAT2 ELISA Kit
  • Other Species Mouse Acat2 ELISA KitRat Acat2 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Acetyl Coenzyme A Acetyltransferase 2 (ACAT2). No significant cross-reactivity or interference between Acetyl Coenzyme A Acetyltransferase 2 (ACAT2) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 31.5pg/mL.
  • Intended UseHuman ACAT2 ELISA Kit allows for the in vitro quantitative determination of ACAT2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Acetyl Coenzyme A Acetyltransferase 2 (ACAT2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Acetyl Coenzyme A Acetyltransferase 2 (ACAT2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Acetyl Coenzyme A Acetyltransferase 2 (ACAT2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Acetyl Coenzyme A Acetyltransferase 2 (ACAT2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Acetyl-CoA acetyltransferase, cytosolic Protein information
  • Uniprot ID THIC_HUMAN
  • Uniprot AC Q9BWD1; B7Z233; E1P5B1; Q16146; Q5TCL7; Q8TDM4;
  • UniGene Hs.571037;
  • GeneID 39
  • KEGG hsa:39;
  • Human Acetyl-CoA acetyltransferase, cytosolic Protein SEQUENCE
  • SEQUENCE 397 AA; 41351 MW; E3A8DAFB6F341B18 CRC64;

    MNAGSDPVVI VSAARTIIGS FNGALAAVPV QDLGSTVIKE VLKRATVAPE

    DVSEVIFGHV LAAGCGQNPV RQASVGAGIP YSVPAWSCQM ICGSGLKAVC

    LAVQSIGIGD SSIVVAGGME NMSKAPHLAY LRTGVKIGEM PLTDSILCDG

    LTDAFHNCHM GITAENVAKK WQVSREDQDK VAVLSQNRTE NAQKAGHFDK

    EIVPVLVSTR KGLIEVKTDE FPRHGSNIEA MSKLKPYFLT DGTGTVTPAN

    ASGINDGAAA VVLMKKSEAD KRGLTPLARI VSWSQVGVEP SIMGIGPIPA

    IKQAVTKAGW SLEDVDIFEI NEAFAAVSAA IVKELGLNPE KVNIEGGAIA

    LGHPLGASGC RILVTLLHTL ERMGRSRGVA ALCIGGGMGI AMCVQRE

  • UCSC uc010kjy.4; human. [Q9BWD1-1];


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