Human DUSP6 ELISA Kit

Alternative name
Human dual specificity protein phosphatase PYST1 ELISA Kit;Human mitogen-activated protein kinase phosphatase 3 ELISA Kit;Human MAP kinase phosphatase 3 ELISA Kit;Human MKP-3 ELISA Kit;Human MKP3 ELISA Kit;Human PYST1 ELISA Kit;Human HH19 ELISA Kit;Human dual specificity phosphatase 6 ELISA Kit;Human dual specificity protein phosphatase 6 ELISA Kit;Human serine/threonine specific protein phosphatase ELISA Kit;
Catalog
E012456
species
Human
GeneDUSP6
SpecificityThis assay has high sensitivity and excellent specificity for detection of Dual Specificity Phosphatase 6 (DUSP6). No significant cross-reactivity or interference between Dual Specificity Phosphatase 6 (DUSP6) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.126ng/mL.
Intended UseHuman DUSP6 ELISA Kit allows for the in vitro quantitative determination of DUSP6 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dual Specificity Phosphatase 6 (DUSP6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dual Specificity Phosphatase 6 (DUSP6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dual Specificity Phosphatase 6 (DUSP6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dual Specificity Phosphatase 6 (DUSP6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Dual specificity protein phosphatase 6 Protein information
Uniprot ID DUS6_HUMAN
Uniprot AC Q16828; O75109; Q53Y75; Q9BSH6;
UniGene Hs.298654; Hs.718640;
GeneID 1848
KEGG hsa:1848;
Human Dual specificity protein phosphatase 6 Protein SEQUENCE
SEQUENCE 381 AA; 42320 MW; 386612227F5D3B2A CRC64;
MIDTLRPVPF ASEMAISKTV AWLNEQLELG NERLLLMDCR PQELYESSHI
ESAINVAIPG IMLRRLQKGN LPVRALFTRG EDRDRFTRRC GTDTVVLYDE
SSSDWNENTG GESVLGLLLK KLKDEGCRAF YLEGGFSKFQ AEFSLHCETN
LDGSCSSSSP PLPVLGLGGL RISSDSSSDI ESDLDRDPNS ATDSDGSPLS
NSQPSFPVEI LPFLYLGCAK DSTNLDVLEE FGIKYILNVT PNLPNLFENA
GEFKYKQIPI SDHWSQNLSQ FFPEAISFID EARGKNCGVL VHCLAGISRS
VTVTVAYLMQ KLNLSMNDAY DIVKMKKSNI SPNFNFMGQL LDFERTLGLS
SPCDNRVPAQ QLYFTTPSNQ NVYQVDSLQS T
UCSC uc001tay.5; human. [Q16828-1];

Category

Human DUSP6 ELISA Kit