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Alternative name
Human adipose tissue-specific secretory factor ELISA Kit;Human ADSF ELISA Kit;Human c/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein ELISA Kit;Human cysteine-rich secreted protein A12-alpha-like 2 ELISA Kit;Human cysteine-rich secreted protein FIZZ3 ELISA Kit;Human FIZZ3 ELISA Kit;Human HXCP1 ELISA Kit;Human RSTN ELISA Kit;Human RETN1 ELISA Kit;Human XCP1 ELISA Kit;Human resistin ELISA Kit;Human C/EBP-epsilon regulated myeloid-specific secreted cysteine-rich protein precursor 1 ELISA Kit;Human found in inflammatory zone 3 ELISA Kit;Human resistin delta2 ELISA Kit;
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Catalog
E033231
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species
Human
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GeneRETN
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SpecificityNatural and recombinant human Resistin
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity< 3pg/ml
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Intended UseHuman RETN ELISA Kit allows for the in vitro quantitative determination of RETN , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageStore the whole ELISA kit at 4℃
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Product Description
specificalPrinciple of the assay: human Resistin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Resistin has been precoated onto 96-well plates. Standards(E.coli,K19-P108) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Resistin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Resistin amount of sample captured in plate.
Background: Resistin is an adipose-derived hormone postulated to link adiposity to insulin resistance.1 Type 2 diabetes,characterized by target-tissue resistance to insulin, is epidemic in industrialized societies and is strongly associated with obesity. Adipocytes secrete a unique signalling molecule, which called resistin (for resistance to insulin), that may be the hormone potentially links obesity to diabetes.2 Resistin is overexpressed in human adipose tissue ofobese individuals and is likely to modulate insulin sensitivity. Resistin is, therefore, a candidate gene for insulin resistance.3 The standard product used in this kit is human Resistin with the molecular mass of 51-53KDa. Thedetected Resistin includes zymogen and active enzyme.
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Human Resistin Protein information
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Uniprot ID
RETN_HUMAN
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Uniprot AC
Q9HD89; D6W649; Q540D9; Q76B53;
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UniGene
Hs.283091;
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GeneID
56729
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KEGG
hsa:56729;
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Human Resistin Protein SEQUENCE
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SEQUENCE 108 AA; 11419 MW; 9B127DFE1699B4F1 CRC64;
MKALCLLLLP VLGLLVSSKT LCSMEEAINE RIQEVAGSLI FRAISSIGLE
CQSVTSRGDL
ATCPRGFAVT GCTCGSACGS WDVRAETTCH CQCAGMDWTG
ARCCRVQP
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UCSC
uc002mhf.2; human. [Q9HD89-1];
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