Human B4GALT1 ELISA Kit

Alternative name
Human beta-1,4-GalTase 1 ELISA Kit;Human beta4Gal-T1 ELISA Kit;Human B4GAL-T1 ELISA Kit;Human UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 1 ELISA Kit;Human UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 1 ELISA Kit;Human Nal synthase ELISA Kit;Human GGTB2 ELISA Kit;Human CDG2D ELISA Kit;Human GT1 ELISA Kit;Human GTB ELISA Kit;Human beta-1,4-galactosyltransferase 1 ELISA Kit;Human UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1 ELISA Kit;Human glycoprotein-4-beta-galactosyltransferase 2 ELISA Kit;Human lactose synthase ELISA Kit;
Catalog
E004642
species
Human
GeneB4GALT1
Standard Curve
Other Species Mouse B4galt1 ELISA Kit Bovine B4GALT1 ELISA Kit Rat B4galt1 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of B4GALT1. No significant cross-reactivity or interference between B4GALT1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between B4GALT1 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 ng/mL.
Intended UseHuman B4GALT1 ELISA Kit allows for the in vitro quantitative determination of B4GALT1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilySignal Transduction
Product Description
specificalPrinciple of the assay: B4GALT1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-B4GALT1 antibody and an B4GALT1-HRP conjugate. The assay sample and buffer are incubated together with B4GALT1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the B4GALT1 concentration since B4GALT1 from samples and B4GALT1-HRP conjugate compete for the anti-B4GALT1 antibody binding site. Since the number of sites is limited, as more sites are occupied by B4GALT1 from the sample, fewer sites are left to bind B4GALT1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The B4GALT1 concentration in each sample is interpolated from this standard curve.
Human Processed beta-1,4-galactosyltransferase 1 Protein information
Uniprot ID B4GT1_HUMAN
Uniprot AC Q14523;
UniGene Hs.272011;
GeneID 2683
KEGG hsa:2683;
Human Processed beta-1,4-galactosyltransferase 1 Protein SEQUENCE
SEQUENCE 398 AA; 43920 MW; 29B224C83C61E165 CRC64;
MRLREPLLSG SAAMPGASLQ RACRLLVAVC ALHLGVTLVY YLAGRDLSRL
PQLVGVSTPL QGGSNSAAAI GQSSGELRTG GARPPPPLGA SSQPRPGGDS
SPVVDSGPGP ASNLTSVPVP HTTALSLPAC PEESPLLVGP MLIEFNMPVD
LELVAKQNPN VKMGGRYAPR DCVSPHKVAI IIPFRNRQEH LKYWLYYLHP
VLQRQQLDYG IYVINQAGDT IFNRAKLLNV GFQEALKDYD YTCFVFSDVD
LIPMNDHNAY RCFSQPRHIS VAMDKFGFSL PYVQYFGGVS ALSKQQFLTI
NGFPNNYWGW GGEDDDIFNR LVFRGMSISR PNAVVGRCRM IRHSRDKKNE
PNPQRFDRIA HTKETMLSDG LNSLTYQVLD VQRYPLYTQI TVDIGTPS
UCSC uc003zsg.3; human. [P15291-1];

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Human B4GALT1 ELISA Kit