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Human PKLR ELISA Kit

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  • Alternative name

    Human pyruvate kinase 1 ELISA Kit;Human pyruvate kinase isozymes L/R ELISA Kit;Human R-type/L-type pyruvate kinase ELISA Kit;Human red cell/liver pyruvate kinase ELISA Kit;Human PK1 ELISA Kit;Human PKL ELISA Kit;Human PKR ELISA Kit;Human PKRL ELISA Kit;Human RPK ELISA Kit;Human pyruvate kinase, liver and RBC ELISA Kit;Human pyruvate kinase PKLR ELISA Kit;Human pyruvate kinase isozyme R/L ELISA Kit;Human pyruvate kinase isozymes R/L ELISA Kit;Human pyruvate kinase type L ELISA Kit;Human pyruvate kinase, liver and blood cell ELISA Kit;
  • Catalog
    E032591
  • species
    Human
  • GenePKLR
  • Other Species Mouse Pklr ELISA KitCanine PKLR ELISA KitRat Pklr ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of human R-PK. No significant cross-reactivity or interference between human R-PK and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.78 mU/ml.
  • Intended UseHuman PKLR ELISA Kit allows for the in vitro quantitative determination of PKLR , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay||This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for R-PK has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any R-PK present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for R-PK is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of R-PK bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human Pyruvate kinase PKLR Protein information
  • Uniprot ID KPYR_HUMAN
  • Uniprot AC P30613; O75758; P11973;
  • UniGene Hs.95990;
  • GeneID 5313
  • KEGG hsa:5313;
  • Human Pyruvate kinase PKLR Protein SEQUENCE

  • SEQUENCE 574 AA; 61830 MW; 3B430896832032F5 CRC64;

    MSIQENISSL QLRSWVSKSQ RDLAKSILIG APGGPAGYLR RASVAQLTQE

    LGTAFFQQQQ LPAAMADTFL EHLCLLDIDS EPVAARSTSI IATIGPASRS

    VERLKEMIKA GMNIARLNFS HGSHEYHAES IANVREAVES FAGSPLSYRP

    VAIALDTKGP EIRTGILQGG PESEVELVKG SQVLVTVDPA FRTRGNANTV

    WVDYPNIVRV VPVGGRIYID DGLISLVVQK IGPEGLVTQV ENGGVLGSRK

    GVNLPGAQVD LPGLSEQDVR DLRFGVEHGV DIVFASFVRK ASDVAAVRAA

    LGPEGHGIKI ISKIENHEGV KRFDEILEVS DGIMVARGDL GIEIPAEKVF

    LAQKMMIGRC NLAGKPVVCA TQMLESMITK PRPTRAETSD VANAVLDGAD

    CIMLSGETAK GNFPVEAVKM QHAIAREAEA AVYHRQLFEE LRRAAPLSRD

    PTEVTAIGAV EAAFKCCAAA IIVLTTTGRS AQLLSRYRPR AAVIAVTRSA

    QAARQVHLCR GVFPLLYREP PEAIWADDVD RRVQFGIESG KLRGFLRVGD

    LVIVVTGWRP GSGYTNIMRV LSIS

  • UCSC uc001fka.5; human. [P30613-1];


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