Human KCNQ1 ELISA Kit

Alternative name
Human IKs producing slow voltage-gated potassium channel subunit alpha KvLQT1 ELISA Kit;Human KQT-like 1 ELISA Kit;Human voltage-gated potassium channel subunit Kv7.1 ELISA Kit;Human ATFB1 ELISA Kit;Human ATFB3 ELISA Kit;Human JLNS1 ELISA Kit;Human KCNA8 ELISA Kit;Human KCNA9 ELISA Kit;Human KVLQT1 ELISA Kit;Human Kv1.9 ELISA Kit;Human Kv7.1 ELISA Kit;Human LQT ELISA Kit;Human LQT1 ELISA Kit;Human RWS ELISA Kit;Human SQT2 ELISA Kit;Human WRS ELISA Kit;Human potassium voltage-gated channel subfamily Q member 1 ELISA Kit;Human potassium voltage-gated channel subfamily KQT member 1 ELISA Kit;Human kidney and cardiac voltage dependend K+ channel ELISA Kit;Human potassium channel, voltage gated KQT-like subfamily Q, member 1 ELISA Kit;Human potassium voltage-gated channel, KQT-like subfamily, member 1 ELISA Kit;Human slow delayed rectifier channel subunit ELISA Kit;
Catalog
E030274
species
Human
GeneKCNQ1
SpecificityThis assay has high sensitivity and excellent specificity for detection of KCNQ1. No significant cross-reactivity or interference between KCNQ1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between KCNQ1 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman KCNQ1 ELISA Kit allows for the in vitro quantitative determination of KCNQ1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalIntended Uses||This KCNQ1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human KCNQ1. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: KCNQ1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-KCNQ1 antibody and an KCNQ1-HRP conjugate. The assay sample and buffer are incubated together with KCNQ1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the KCNQ1 concentration since KCNQ1 from samples and KCNQ1-HRP conjugate compete for the anti-KCNQ1 antibody binding site. Since the number of sites is limited, as more sites are occupied by KCNQ1 from the sample, fewer sites are left to bind KCNQ1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The KCNQ1 concentration in each sample is interpolated from this standard curve.
Human Potassium voltage-gated channel subfamily KQT member 1 Protein information
Uniprot ID KCNQ1_HUMAN
Uniprot AC Q9UMN9;
UniGene Hs.95162;
GeneID 3784
KEGG hsa:3784;
Human Potassium voltage-gated channel subfamily KQT member 1 Protein SEQUENCE
SEQUENCE 676 AA; 74699 MW; ADFCA9E2B9763B21 CRC64;
MAAASSPPRA ERKRWGWGRL PGARRGSAGL AKKCPFSLEL AEGGPAGGAL
YAPIAPGAPG PAPPASPAAP AAPPVASDLG PRPPVSLDPR VSIYSTRRPV
LARTHVQGRV YNFLERPTGW KCFVYHFAVF LIVLVCLIFS VLSTIEQYAA
LATGTLFWME IVLVVFFGTE YVVRLWSAGC RSKYVGLWGR LRFARKPISI
IDLIVVVASM VVLCVGSKGQ VFATSAIRGI RFLQILRMLH VDRQGGTWRL
LGSVVFIHRQ ELITTLYIGF LGLIFSSYFV YLAEKDAVNE SGRVEFGSYA
DALWWGVVTV TTIGYGDKVP QTWVGKTIAS CFSVFAISFF ALPAGILGSG
FALKVQQKQR QKHFNRQIPA AASLIQTAWR CYAAENPDSS TWKIYIRKAP
RSHTLLSPSP KPKKSVVVKK KKFKLDKDNG VTPGEKMLTV PHITCDPPEE
RRLDHFSVDG YDSSVRKSPT LLEVSMPHFM RTNSFAEDLD LEGETLLTPI
THISQLREHH RATIKVIRRM QYFVAKKKFQ QARKPYDVRD VIEQYSQGHL
NLMVRIKELQ RRLDQSIGKP SLFISVSEKS KDRGSNTIGA RLNRVEDKVT
QLDQRLALIT DMLHQLLSLH GGSTPGSGGP PREGGAHITQ PCGSGGSVDP
ELFLPSNTLP TYEQLTVPRR GPDEGS
UCSC uc001lwn.4; human. [P51787-1];

Category

Human KCNQ1 ELISA Kit