Human CPT2 ELISA Kit

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  • Alternative name

    Human carnitine palmitoyltransferase II ELISA Kit;Human CPT II ELISA Kit;Human CPT1 ELISA Kit;Human CPTASE ELISA Kit;Human IIAE4 ELISA Kit;Human carnitine palmitoyltransferase 2 ELISA Kit;Human carnitine O-palmitoyltransferase 2, mitochondrial ELISA Kit;Human testicular secretory protein Li 13 ELISA Kit;

  • Catalog
    E006736
  • species
    Human
  • GeneCPT2
  • Other Species Mouse Cpt2 ELISA KitBovine CPT2 ELISA KitMonkey CPT2 ELISA KitRat Cpt2 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of CPT2. No significant cross-reactivity or interference between CPT2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CPT2 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman CPT2 ELISA Kit allows for the in vitro quantitative determination of CPT2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Intended Uses: This CPT2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CPT2. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||CPT2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-CPT2 antibody and an CPT2-HRP conjugate. The assay sample and buffer are incubated together with CPT2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CPT2 concentration since CPT2 from samples and CPT2-HRP conjugate compete for the anti-CPT2 antibody binding site. Since the number of sites is limited, as more sites are occupied by CPT2 from the sample, fewer sites are left to bind CPT2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CPT2 concentration in each sample is interpolated from this standard curve.
  • Human Carnitine O-palmitoyltransferase 2, mitochondrial Protein information
  • Uniprot ID CPT2_HUMAN
  • Uniprot AC P23786; B2R6S0; Q5SW68; Q9BQ26;
  • UniGene Hs.713535;
  • GeneID 1376
  • KEGG hsa:1376;
  • Human Carnitine O-palmitoyltransferase 2, mitochondrial Protein SEQUENCE
  • SEQUENCE 658 AA; 73777 MW; 6444B75ACD57140F CRC64;

    MVPRLLLRAW PRGPAVGPGA PSRPLSAGSG PGQYLQRSIV PTMHYQDSLP

    RLPIPKLEDT IRRYLSAQKP LLNDGQFRKT EQFCKSFENG IGKELHEQLV

    ALDKQNKHTS YISGPWFDMY LSARDSVVLN FNPFMAFNPD PKSEYNDQLT

    RATNMTVSAI RFLKTLRAGL LEPEVFHLNP AKSDTITFKR LIRFVPSSLS

    WYGAYLVNAY PLDMSQYFRL FNSTRLPKPS RDELFTDDKA RHLLVLRKGN

    FYIFDVLDQD GNIVSPSEIQ AHLKYILSDS SPAPEFPLAY LTSENRDIWA

    ELRQKLMSSG NEESLRKVDS AVFCLCLDDF PIKDLVHLSH NMLHGDGTNR

    WFDKSFNLII AKDGSTAVHF EHSWGDGVAV LRFFNEVFKD STQTPAVTPQ

    SQPATTDSTV TVQKLNFELT DALKTGITAA KEKFDATMKT LTIDCVQFQR

    GGKEFLKKQK LSPDAVAQLA FQMAFLRQYG QTVATYESCS TAAFKHGRTE

    TIRPASVYTK RCSEAFVREP SRHSAGELQQ MMVECSKYHG QLTKEAAMGQ

    GFDRHLFALR HLAAAKGIIL PELYLDPAYG QINHNVLSTS TLSSPAVNLG

    GFAPVVSDGF GVGYAVHDNW IGCNVSSYPG RNAREFLQCV EKALEDMFDA

    LEGKSIKS

  • UCSC uc001cvb.4; human.;



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