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Human RPTOR ELISA Kit

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  • Alternative name

    Human raptor ELISA Kit;Human p150 target of rapamycin (TOR)-scaffold protein ELISA Kit;Human KIAA1303 ELISA Kit;Human KOG1 ELISA Kit;Human Mip1 ELISA Kit;Human regulatory associated protein of MTOR complex 1 ELISA Kit;Human regulatory-associated protein of mTOR ELISA Kit;Human p150 target of rapamycin (TOR)-scaffold protein containing WD-repeats ELISA Kit;
  • Catalog
    E033146
  • species
    Human
  • GeneRPTOR
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity<0.188ng/ml
  • Intended UseHuman RPTOR ELISA Kit allows for the in vitro quantitative determination of RPTOR , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-RPTOR antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-RPTOR antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the RPTOR amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of RPTOR can be calculated.
  • Human Regulatory-associated protein of mTOR Protein information
  • Uniprot ID RPTOR_HUMAN
  • Uniprot AC Q8N122; B2RN36; C6KEF2; F5H7J5; Q8N4V9; Q8TB32; Q9P2P3;
  • UniGene Hs.133044;
  • GeneID 57521
  • KEGG hsa:57521;
  • Human Regulatory-associated protein of mTOR Protein SEQUENCE

  • SEQUENCE 1335 AA; 149038 MW; 688ED1943F45045A CRC64;

    MESEMLQSPL LGLGEEDEAD LTDWNLPLAF MKKRHCEKIE GSKSLAQSWR

    MKDRMKTVSV ALVLCLNVGV DPPDVVKTTP CARLECWIDP LSMGPQKALE

    TIGANLQKQY ENWQPRARYK QSLDPTVDEV KKLCTSLRRN AKEERVLFHY

    NGHGVPRPTV NGEVWVFNKN YTQYIPLSIY DLQTWMGSPS IFVYDCSNAG

    LIVKSFKQFA LQREQELEVA AINPNHPLAQ MPLPPSMKNC IQLAACEATE

    LLPMIPDLPA DLFTSCLTTP IKIALRWFCM QKCVSLVPGV TLDLIEKIPG

    RLNDRRTPLG ELNWIFTAIT DTIAWNVLPR DLFQKLFRQD LLVASLFRNF

    LLAERIMRSY NCTPVSSPRL PPTYMHAMWQ AWDLAVDICL SQLPTIIEEG

    TAFRHSPFFA EQLTAFQVWL TMGVENRNPP EQLPIVLQVL LSQVHRLRAL

    DLLGRFLDLG PWAVSLALSV GIFPYVLKLL QSSARELRPL LVFIWAKILA

    VDSSCQADLV KDNGHKYFLS VLADPYMPAE HRTMTAFILA VIVNSYHTGQ

    EACLQGNLIA ICLEQLNDPH PLLRQWVAIC LGRIWQNFDS ARWCGVRDSA

    HEKLYSLLSD PIPEVRCAAV FALGTFVGNS AERTDHSTTI DHNVAMMLAQ

    LVSDGSPMVR KELVVALSHL VVQYESNFCT VALQFIEEEK NYALPSPATT

    EGGSLTPVRD SPCTPRLRSV SSYGNIRAVA TARSLNKSLQ NLSLTEESGG

    AVAFSPGNLS TSSSASSTLG SPENEEHILS FETIDKMRRA SSYSSLNSLI

    GVSFNSVYTQ IWRVLLHLAA DPYPEVSDVA MKVLNSIAYK ATVNARPQRV

    LDTSSLTQSA PASPTNKGVH IHQAGGSPPA SSTSSSSLTN DVAKQPVSRD

    LPSGRPGTTG PAGAQYTPHS HQFPRTRKMF DKGPEQTADD ADDAAGHKSF

    ISATVQTGFC DWSARYFAQP VMKIPEEHDL ESQIRKEREW RFLRNSRVRR

    QAQQVIQKGI TRLDDQIFLN RNPGVPSVVK FHPFTPCIAV ADKDSICFWD

    WEKGEKLDYF HNGNPRYTRV TAMEYLNGQD CSLLLTATDD GAIRVWKNFA

    DLEKNPEMVT AWQGLSDMLP TTRGAGMVVD WEQETGLLMS SGDVRIVRIW

    DTDREMKVQD IPTGADSCVT SLSCDSHRSL IVAGLGDGSI RVYDRRMALS

    ECRVMTYREH TAWVVKASLQ KRPDGHIVSV SVNGDVRIFD PRMPESVNVL

    QIVKGLTALD IHPQADLIAC GSVNQFTAIY NSSGELINNI KYYDGFMGQR

    VGAISCLAFH PHWPHLAVGS NDYYISVYSV EKRVR

  • UCSC uc002jys.5; human. [Q8N122-1];



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