Mouse SPH ELISA Kit

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  • Catalog
    E068742
  • species
    Mouse
  • GeneSPH
  • Standard CurveMouse SPH ELISA Kit
  • Other Species Human SM/SPH ELISA KitHuman SPH ELISA KitBovine SPH ELISA KitCanine SPH ELISA KitChicken SPH ELISA KitGeneral SPH ELISA KitPorcine SPH ELISA KitRabbit SPH ELISA KitRat SPH ELISA KitCamel SPH ELISA KitGoat SPH ELISA KitGuinea Pig SPH ELISA KitHamster SPH ELISA KitHorse SPH ELISA KitMonkey SPH ELISA KitSheep SPH ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Sphingomyelin (SPH). No significant cross-reactivity or interference between Sphingomyelin (SPH) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 1.65ug/mL.
  • Intended UseMouse SPH ELISA Kit allows for the in vitro quantitative determination of SPH , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Sphingomyelin (SPH) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Sphingomyelin (SPH) and unlabeled Sphingomyelin (SPH) (Standards or samples) with the pre-coated antibody specific to Sphingomyelin (SPH). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Sphingomyelin (SPH) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Sphingomyelin (SPH) in the sample.


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