Human PDHB ELISA Kit

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  • Alternative name

    Human PDHE1-B ELISA Kit;Human PHE1B ELISA Kit;Human PDHBD ELISA Kit;Human pyruvate dehydrogenase (lipoamide) beta ELISA Kit;Human pyruvate dehydrogenase E1 component subunit beta, mitochondrial ELISA Kit;Human pyruvate dehydrogenase, E1 beta polypeptide ELISA Kit;

  • Catalog
    E032564
  • species
    Human
  • GenePDHB
  • Other Species Mouse Pdhb ELISA KitBovine PDHB ELISA KitRat Pdhb ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Pyruvate Dehydrogenase Beta (PDHb). No significant cross-reactivity or interference between Pyruvate Dehydrogenase Beta (PDHb) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 1.2 U/L.
  • Intended UseHuman PDHB ELISA Kit allows for the in vitro quantitative determination of PDHB , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pyruvate Dehydrogenase Beta (PDHb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Pyruvate Dehydrogenase Beta (PDHb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pyruvate Dehydrogenase Beta (PDHb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pyruvate Dehydrogenase Beta (PDHb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Pyruvate dehydrogenase E1 component subunit beta, mitochondrial Protein information
  • Uniprot ID ODPB_HUMAN
  • Uniprot AC P11177; B2R7L0; B4DDD7; Q6FH45; Q9BQ27; Q9UFK3;
  • UniGene Hs.161357;
  • GeneID 5162
  • KEGG hsa:5162;
  • Human Pyruvate dehydrogenase E1 component subunit beta, mitochondrial Protein SEQUENCE
  • SEQUENCE 359 AA; 39233 MW; AB459B1259FBDBD3 CRC64;

    MAAVSGLVRR PLREVSGLLK RRFHWTAPAA LQVTVRDAIN QGMDEELERD

    EKVFLLGEEV AQYDGAYKVS RGLWKKYGDK RIIDTPISEM GFAGIAVGAA

    MAGLRPICEF MTFNFSMQAI DQVINSAAKT YYMSGGLQPV PIVFRGPNGA

    SAGVAAQHSQ CFAAWYGHCP GLKVVSPWNS EDAKGLIKSA IRDNNPVVVL

    ENELMYGVPF EFPPEAQSKD FLIPIGKAKI ERQGTHITVV SHSRPVGHCL

    EAAAVLSKEG VECEVINMRT IRPMDMETIE ASVMKTNHLV TVEGGWPQFG

    VGAEICARIM EGPAFNFLDA PAVRVTGADV PMPYAKILED NSIPQVKDII

    FAIKKTLNI

  • UCSC uc003dkf.4; human. [P11177-1];


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