Human PLD2 ELISA Kit

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  • Alternative name

    Human PLD 2 ELISA Kit;Human hPLD2 ELISA Kit;Human choline phosphatase 2 ELISA Kit;Human PLD1C ELISA Kit;Human phosphatidylcholine-hydrolyzing phospholipase D2 ELISA Kit;Human phospholipase D2 ELISA Kit;

  • Catalog
    E029238
  • species
    Human
  • GenePLD2
  • Standard CurveHuman PLD2 ELISA Kit
  • Other Species Mouse Pld2 ELISA KitBovine PLD2 ELISA KitRat Pld2 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Phospholipase D2 (PLD2). No significant cross-reactivity or interference between Phospholipase D2 (PLD2) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.55ng/mL.
  • Intended UseHuman PLD2 ELISA Kit allows for the in vitro quantitative determination of PLD2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phospholipase D2 (PLD2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phospholipase D2 (PLD2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phospholipase D2 (PLD2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phospholipase D2 (PLD2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Phospholipase D2 Protein information
  • Uniprot ID PLD2_HUMAN
  • Uniprot AC O14939; I3L2C9; O43540; O43579; O43580; Q6PGR0; Q96BY3;
  • UniGene Hs.104519;
  • GeneID 5338
  • KEGG hsa:5338;
  • Human Phospholipase D2 Protein SEQUENCE
  • SEQUENCE 933 AA; 105987 MW; F25F6B73B45F57ED CRC64;

    MTATPESLFP TGDELDSSQL QMESDEVDTL KEGEDPADRM HPFLAIYELQ

    SLKVHPLVFA PGVPVTAQVV GTERYTSGSK VGTCTLYSVR LTHGDFSWTT

    KKKYRHFQEL HRDLLRHKVL MSLLPLARFA VAYSPARDAG NREMPSLPRA

    GPEGSTRHAA SKQKYLENYL NRLLTMSFYR NYHAMTEFLE VSQLSFIPDL

    GRKGLEGMIR KRSGGHRVPG LTCCGRDQVC YRWSKRWLVV KDSFLLYMCL

    ETGAISFVQL FDPGFEVQVG KRSTEARHGV RIDTSHRSLI LKCSSYRQAR

    WWAQEITELA QGPGRDFLQL HRHDSYAPPR PGTLARWFVN GAGYFAAVAD

    AILRAQEEIF ITDWWLSPEV YLKRPAHSDD WRLDIMLKRK AEEGVRVSIL

    LFKEVELALG INSGYSKRAL MLLHPNIKVM RHPDQVTLWA HHEKLLVVDQ

    VVAFLGGLDL AYGRWDDLHY RLTDLGDSSE SAASQPPTPR PDSPATPDLS

    HNQFFWLGKD YSNLITKDWV QLDRPFEDFI DRETTPRMPW RDVGVVVHGL

    PARDLARHFI QRWNFTKTTK AKYKTPTYPY LLPKSTSTAN QLPFTLPGGQ

    CTTVQVLRSV DRWSAGTLEN SILNAYLHTI RESQHFLYIE NQFFISCSDG

    RTVLNKVGDE IVDRILKAHK QGWCYRVYVL LPLLPGFEGD ISTGGGNSIQ

    AILHFTYRTL CRGEYSILHR LKAAMGTAWR DYISICGLRT HGELGGHPVS

    ELIYIHSKVL IADDRTVIIG SANINDRSLL GKRDSELAVL IEDTETEPSL

    MNGAEYQAGR FALSLRKHCF GVILGANTRP DLDLRDPICD DFFQLWQDMA

    ESNANIYEQI FRCLPSNATR SLRTLREYVA VEPLATVSPP LARSELTQVQ

    GHLVHFPLKF LEDESLLPPL GSKEGMIPLE VWT

  • UCSC uc002fzc.4; human. [O14939-1];


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