Mouse Gltp ELISA Kit

Alternative name
Mouse GLTP ELISA Kit;Mouse 1110001F24Rik ELISA Kit;Mouse C76925 ELISA Kit;Mouse C77564 ELISA Kit;Mouse glycolipid transfer protein ELISA Kit;
Catalog
E054624
species
Mouse
GeneGltp
SpecificityThis assay has high sensitivity and excellent specificity for detection of GLTP. No significant cross-reactivity or interference between GLTP and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GLTP and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseMouse Gltp ELISA Kit allows for the in vitro quantitative determination of Gltp , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: GLTP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GLTP antibody and an GLTP-HRP conjugate. The assay sample and buffer are incubated together with GLTP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GLTP concentration since GLTP from samples and GLTP-HRP conjugate compete for the anti-GLTP antibody binding site. Since the number of sites is limited, as more sites are occupied by GLTP from the sample, fewer sites are left to bind GLTP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GLTP concentration in each sample is interpolated from this standard curve.
Mouse Glycolipid transfer protein Protein information
Uniprot ID GLTP_MOUSE
Uniprot AC Q9JL62; Q91YJ7; Q9CTK9;
UniGene Mm.275766;
GeneID 56356
KEGG mmu:56356;
Mouse Glycolipid transfer protein Protein SEQUENCE
SEQUENCE 209 AA; 23690 MW; A7CBDD142C76BB1A CRC64;
MALLAEHLLK PLPADRQIET GPFLEAVAHL PPFFDCLGSP VFTPIKADIS
GNITKIKAVY DTDPAKFKTL QNILEVEKGM YGAEWPKVGA TLALLWLKRG
LRFIQVFLQS ICDGERDENH PNLIRVNANK AYEMALKKYH GWLVQKIFKA
ALYAAPYKSD FLKALSKGQN VTEEECLEKI RLFLVNYTAT IDAIYDMYTK
MNAELDYTV
UCSC uc008yzw.2; mouse.;

Category

Mouse Gltp ELISA Kit

Mouse GLTP ELISA Kit;Mouse 1110001F24Rik ELISA Kit;Mouse C76925 ELISA Kit;Mouse C77564 ELISA Kit;Mouse glycolipid transfer protein ELISA Kit;