Mouse Gltp ELISA Kit

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  • Alternative name

    Mouse GLTP ELISA Kit;Mouse 1110001F24Rik ELISA Kit;Mouse C76925 ELISA Kit;Mouse C77564 ELISA Kit;Mouse glycolipid transfer protein ELISA Kit;

  • Catalog
    E054624
  • species
    Mouse
  • GeneGltp
  • Standard CurveMouse Gltp ELISA Kit
  • Other Species Human GLTP ELISA KitBovine GLTP ELISA KitPorcine GLTP ELISA KitRat GLTP ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of GLTP. No significant cross-reactivity or interference between GLTP and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GLTP and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseMouse Gltp ELISA Kit allows for the in vitro quantitative determination of Gltp , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: GLTP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GLTP antibody and an GLTP-HRP conjugate. The assay sample and buffer are incubated together with GLTP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GLTP concentration since GLTP from samples and GLTP-HRP conjugate compete for the anti-GLTP antibody binding site. Since the number of sites is limited, as more sites are occupied by GLTP from the sample, fewer sites are left to bind GLTP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GLTP concentration in each sample is interpolated from this standard curve.
  • Mouse Glycolipid transfer protein Protein information
  • Uniprot ID GLTP_MOUSE
  • Uniprot AC Q9JL62; Q91YJ7; Q9CTK9;
  • UniGene Mm.275766;
  • GeneID 56356
  • KEGG mmu:56356;
  • Mouse Glycolipid transfer protein Protein SEQUENCE
  • SEQUENCE 209 AA; 23690 MW; A7CBDD142C76BB1A CRC64;

    MALLAEHLLK PLPADRQIET GPFLEAVAHL PPFFDCLGSP VFTPIKADIS

    GNITKIKAVY DTDPAKFKTL QNILEVEKGM YGAEWPKVGA TLALLWLKRG

    LRFIQVFLQS ICDGERDENH PNLIRVNANK AYEMALKKYH GWLVQKIFKA

    ALYAAPYKSD FLKALSKGQN VTEEECLEKI RLFLVNYTAT IDAIYDMYTK

    MNAELDYTV

  • UCSC uc008yzw.2; mouse.;


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