Human PCDH1 ELISA Kit

Alternative name
Human cadherin-like protein 1 ELISA Kit;Human Protocadherin-42 ELISA Kit;Human PC42 ELISA Kit;Human PCDH42 ELISA Kit;Human protocadherin 1 ELISA Kit;Human protocadherin-1 ELISA Kit;Human cadherin-like 1 ELISA Kit;Human protocadherin 42 ELISA Kit;
Catalog
E032085
species
Human
GenePCDH1
SpecificityThis assay has high sensitivity and excellent specificity for detection of PFN. No significant cross-reactivity or interference between PFN and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PFN and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 ng/mL.
Intended UseHuman PCDH1 ELISA Kit allows for the in vitro quantitative determination of PCDH1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: PFN ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PFN antibody and an PFN-HRP conjugate. The assay sample and buffer are incubated together with PFN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PFN concentration since PFN from samples and PFN-HRP conjugate compete for the anti-PFN antibody binding site. Since the number of sites is limited, as more sites are occupied by PFN from the sample, fewer sites are left to bind PFN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PFN concentration in each sample is interpolated from this standard curve.
Human Protocadherin-1 Protein information
Uniprot ID PCDH1_HUMAN
Uniprot AC Q08174; Q8IUP2;
UniGene Hs.79769;
GeneID 5097
KEGG hsa:5097;
Human Protocadherin-1 Protein SEQUENCE
SEQUENCE 1060 AA; 114743 MW; 0F4F24F3EE607D04 CRC64;
MDSGAGGRRC PEAALLILGP PRMEHLRHSP GPGGQRLLLP SMLLALLLLL
APSPGHATRV VYKVPEEQPP NTLIGSLAAD YGFPDVGHLY KLEVGAPYLR
VDGKTGDIFT TETSIDREGL RECQNQLPGD PCILEFEVSI TDLVQNGSPR
LLEGQIEVQD INDNTPNFAS PVITLAIPEN TNIGSLFPIP LASDRDAGPN
GVASYELQAG PEAQELFGLQ VAEDQEEKQP QLIVMGNLDR ERWDSYDLTI
KVQDGGSPPR ASSALLRVTV LDTNDNAPKF ERPSYEAELS ENSPIGHSVI
QVKANDSDQG ANAEIEYTFH QAPEVVRRLL RLDRNTGLIT VQGPVDREDL
STLRFSVLAK DRGTNPKSAR AQVVVTVKDM NDNAPTIEIR GIGLVTHQDG
MANISEDVAE ETAVALVQVS DRDEGENAAV TCVVAGDVPF QLRQASETGS
DSKKKYFLQT TTPLDYEKVK DYTIEIVAVD SGNPPLSSTN SLKVQVVDVN
DNAPVFTQSV TEVAFPENNK PGEVIAEITA SDADSGSNAE LVYSLEPEPA
AKGLFTISPE TGEIQVKTSL DREQRESYEL KVVAADRGSP SLQGTATVLV
NVLDCNDNDP KFMLSGYNFS VMENMPALSP VGMVTVIDGD KGENAQVQLS
VEQDNGDFVI QNGTGTILSS LSFDREQQST YTFQLKAVDG GVPPRSAYVG
VTINVLDEND NAPYITAPSN TSHKLLTPQT RLGETVSQVA AEDFDSGVNA
ELIYSIAGGN PYGLFQIGSH SGAITLEKEI ERRHHGLHRL VVKVSDRGKP
PRYGTALVHL YVNETLANRT LLETLLGHSL DTPLDIDIAG DPEYERSKQR
GNILFGVVAG VVAVALLIAL AVLVRYCRQR EAKSGYQAGK KETKDLYAPK
PSGKASKGNK SKGKKSKSPK PVKPVEDEDE AGLQKSLKFN LMSDAPGDSP
RIHLPLNYPP GSPDLGRHYR SNSPLPSIQL QPQSPSASKK HQVVQDLPPA
NTFVGTGDTT STGSEQYSDY SYRTNPPKYP SKQVGQPFQL STPQPLPHPY
HGAIWTEVWE
UCSC uc003llp.4; human. [Q08174-1];

Category

Human PCDH1 ELISA Kit

Human cadherin-like protein 1 ELISA Kit;Human Protocadherin-42 ELISA Kit;Human PC42 ELISA Kit;Human PCDH42 ELISA Kit;Human protocadherin 1 ELISA Kit;Human protocadherin-1 ELISA Kit;Human cadherin-like 1 ELISA Kit;Human protocadherin 42 ELISA Kit;