Human HRG ELISA Kit

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  • Alternative name

    Human histidine-proline-rich glycoprotein ELISA Kit;Human HPRG ELISA Kit;Human HRGP ELISA Kit;Human THPH11 ELISA Kit;Human histidine rich glycoprotein ELISA Kit;Human histidine-rich glycoprotein ELISA Kit;

  • Catalog
    E018716
  • species
    Human
  • GeneHRG
  • Standard CurveHuman HRG ELISA Kit
  • Other Species Mouse Hrg ELISA KitBovine HRG ELISA KitRabbit HRG ELISA KitRat Hrg ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Histidine Rich Glycoprotein (HRG). No significant cross-reactivity or interference between Histidine Rich Glycoprotein (HRG) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.21ng/mL.
  • Intended UseHuman HRG ELISA Kit allows for the in vitro quantitative determination of HRG , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Histidine Rich Glycoprotein (HRG). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Histidine Rich Glycoprotein (HRG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Histidine Rich Glycoprotein (HRG), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Histidine Rich Glycoprotein (HRG) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Histidine-rich glycoprotein Protein information
  • Uniprot ID HRG_HUMAN
  • Uniprot AC P04196; B9EK35; D3DNU7;
  • UniGene Hs.1498;
  • GeneID 3273
  • KEGG hsa:3273;
  • Human Histidine-rich glycoprotein Protein SEQUENCE
  • SEQUENCE 525 AA; 59578 MW; A2B124D6CE93114F CRC64;

    MKALIAALLL ITLQYSCAVS PTDCSAVEPE AEKALDLINK RRRDGYLFQL

    LRIADAHLDR VENTTVYYLV LDVQESDCSV LSRKYWNDCE PPDSRRPSEI

    VIGQCKVIAT RHSHESQDLR VIDFNCTTSS VSSALANTKD SPVLIDFFED

    TERYRKQANK ALEKYKEEND DFASFRVDRI ERVARVRGGE GTGYFVDFSV

    RNCPRHHFPR HPNVFGFCRA DLFYDVEALD LESPKNLVIN CEVFDPQEHE

    NINGVPPHLG HPFHWGGHER SSTTKPPFKP HGSRDHHHPH KPHEHGPPPP

    PDERDHSHGP PLPQGPPPLL PMSCSSCQHA TFGTNGAQRH SHNNNSSDLH

    PHKHHSHEQH PHGHHPHAHH PHEHDTHRQH PHGHHPHGHH PHGHHPHGHH

    PHGHHPHCHD FQDYGPCDPP PHNQGHCCHG HGPPPGHLRR RGPGKGPRPF

    HCRQIGSVYR LPPLRKGEVL PLPEANFPSF PLPHHKHPLK PDNQPFPQSV

    SESCPGKFKS GFPQVSMFFT HTFPK

  • UCSC uc003fqq.5; human.;


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