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Human BRCA1 ELISA Kit

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  • Alternative name

    Human RING finger protein 53 ELISA Kit;Human RING-type E3 ubiquitin transferase BRCA1 ELISA Kit;Human RNF53 ELISA Kit;Human BRCAI ELISA Kit;Human BRCC1 ELISA Kit;Human BROVCA1 ELISA Kit;Human FANCS ELISA Kit;Human IRIS ELISA Kit;Human PNCA4 ELISA Kit;Human PPP1R53 ELISA Kit;Human PSCP ELISA Kit;Human BRCA1, DNA repair associated ELISA Kit;Human breast cancer type 1 susceptibility protein ELISA Kit;Human BRCA1/BRCA2-containing complex, subunit 1 ELISA Kit;Human Fanconi anemia, complementation group S ELISA Kit;Human breast and ovarian cancer susceptibility protein 1 ELISA Kit;Human breast cancer 1, early onset ELISA Kit;Human early onset breast cancer 1 ELISA Kit;Human protein phosphatase 1, regulatory subunit 53 ELISA Kit;
  • Catalog
    E005282
  • species
    Human
  • GeneBRCA1
  • Other Species Mouse Brca1 ELISA KitBovine BRCA1 ELISA KitCanine BRCA1 ELISA KitRat Brca1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Human BRCA1. No significant cross-reactivity or interference between Human BRCA1 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity7.8pg/ml
  • Intended UseHuman BRCA1 ELISA Kit allows for the in vitro quantitative determination of BRCA1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to BRCA-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for BRCA-1. and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain BRCA-1., biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of BRCA-1. in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Breast cancer type 1 susceptibility protein Protein information
  • Uniprot ID BRCA1_HUMAN
  • Uniprot AC P38398; E9PFZ0; O15129; Q1RMC1; Q3LRJ0; Q3LRJ6; Q6IN79; Q7KYU9;
  • UniGene Hs.194143;
  • GeneID 672
  • KEGG hsa:672;
  • Human Breast cancer type 1 susceptibility protein Protein SEQUENCE

  • SEQUENCE 1863 AA; 207721 MW; 89C6D83FF56312AF CRC64;

    MDLSALRVEE VQNVINAMQK ILECPICLEL IKEPVSTKCD HIFCKFCMLK

    LLNQKKGPSQ CPLCKNDITK RSLQESTRFS QLVEELLKII CAFQLDTGLE

    YANSYNFAKK ENNSPEHLKD EVSIIQSMGY RNRAKRLLQS EPENPSLQET

    SLSVQLSNLG TVRTLRTKQR IQPQKTSVYI ELGSDSSEDT VNKATYCSVG

    DQELLQITPQ GTRDEISLDS AKKAACEFSE TDVTNTEHHQ PSNNDLNTTE

    KRAAERHPEK YQGSSVSNLH VEPCGTNTHA SSLQHENSSL LLTKDRMNVE

    KAEFCNKSKQ PGLARSQHNR WAGSKETCND RRTPSTEKKV DLNADPLCER

    KEWNKQKLPC SENPRDTEDV PWITLNSSIQ KVNEWFSRSD ELLGSDDSHD

    GESESNAKVA DVLDVLNEVD EYSGSSEKID LLASDPHEAL ICKSERVHSK

    SVESNIEDKI FGKTYRKKAS LPNLSHVTEN LIIGAFVTEP QIIQERPLTN

    KLKRKRRPTS GLHPEDFIKK ADLAVQKTPE MINQGTNQTE QNGQVMNITN

    SGHENKTKGD SIQNEKNPNP IESLEKESAF KTKAEPISSS ISNMELELNI

    HNSKAPKKNR LRRKSSTRHI HALELVVSRN LSPPNCTELQ IDSCSSSEEI

    KKKKYNQMPV RHSRNLQLME GKEPATGAKK SNKPNEQTSK RHDSDTFPEL

    KLTNAPGSFT KCSNTSELKE FVNPSLPREE KEEKLETVKV SNNAEDPKDL

    MLSGERVLQT ERSVESSSIS LVPGTDYGTQ ESISLLEVST LGKAKTEPNK

    CVSQCAAFEN PKGLIHGCSK DNRNDTEGFK YPLGHEVNHS RETSIEMEES

    ELDAQYLQNT FKVSKRQSFA PFSNPGNAEE ECATFSAHSG SLKKQSPKVT

    FECEQKEENQ GKNESNIKPV QTVNITAGFP VVGQKDKPVD NAKCSIKGGS

    RFCLSSQFRG NETGLITPNK HGLLQNPYRI PPLFPIKSFV KTKCKKNLLE

    ENFEEHSMSP EREMGNENIP STVSTISRNN IRENVFKEAS SSNINEVGSS

    TNEVGSSINE IGSSDENIQA ELGRNRGPKL NAMLRLGVLQ PEVYKQSLPG

    SNCKHPEIKK QEYEEVVQTV NTDFSPYLIS DNLEQPMGSS HASQVCSETP

    DDLLDDGEIK EDTSFAENDI KESSAVFSKS VQKGELSRSP SPFTHTHLAQ

    GYRRGAKKLE SSEENLSSED EELPCFQHLL FGKVNNIPSQ STRHSTVATE

    CLSKNTEENL LSLKNSLNDC SNQVILAKAS QEHHLSEETK CSASLFSSQC

    SELEDLTANT NTQDPFLIGS SKQMRHQSES QGVGLSDKEL VSDDEERGTG

    LEENNQEEQS MDSNLGEAAS GCESETSVSE DCSGLSSQSD ILTTQQRDTM

    QHNLIKLQQE MAELEAVLEQ HGSQPSNSYP SIISDSSALE DLRNPEQSTS

    EKAVLTSQKS SEYPISQNPE GLSADKFEVS ADSSTSKNKE PGVERSSPSK

    CPSLDDRWYM HSCSGSLQNR NYPSQEELIK VVDVEEQQLE ESGPHDLTET

    SYLPRQDLEG TPYLESGISL FSDDPESDPS EDRAPESARV GNIPSSTSAL

    KVPQLKVAES AQSPAAAHTT DTAGYNAMEE SVSREKPELT ASTERVNKRM

    SMVVSGLTPE EFMLVYKFAR KHHITLTNLI TEETTHVVMK TDAEFVCERT

    LKYFLGIAGG KWVVSYFWVT QSIKERKMLN EHDFEVRGDV VNGRNHQGPK

    RARESQDRKI FRGLEICCYG PFTNMPTDQL EWMVQLCGAS VVKELSSFTL

    GTGVHPIVVV QPDAWTEDNG FHAIGQMCEA PVVTREWVLD SVALYQCQEL

    DTYLIPQIPH SHY

  • UCSC uc010cyx.4; human.;


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