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Alternative name
Phospholipase D1 ELISA KIT; Choline phosphatase 1 ELISA KIT; Phosphatidylcholine-hydrolyzing phospholipase D1PLD1 ELISA KIT; PLD 1 ELISA KIT; hPLD1 ELISA KIT
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Catalog
E029233
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species
Human
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GenePLD1
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SpecificityThis assay has high sensitivity and excellent specificity for detection of human PLD1. No significant cross-reactivity or interference between human PLD1 and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityThe minimum detectable dose of human PLD1 is typically less than 6.25 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mea
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Intended UseHuman PLD1 ELISA Kit allows for the in vitro quantitative determination of PLD1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PLD1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PLD1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLD1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLD1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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