Human MTBP ELISA Kit

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  • Alternative name

    Human hMTBP ELISA Kit;Human MDM2BP ELISA Kit;Human MDM2 binding protein ELISA Kit;Human mdm2-binding protein ELISA Kit;Human MDM2 (mouse double minute 2)-binding protein, 104kD ELISA Kit;Human Mdm2, transformed 3T3 cell double minute 2, p53 binding protein binding protein, 104kDa ELISA Kit;

  • Catalog
    E023894
  • species
    Human
  • GeneMTBP
  • Other Species Mouse Mtbp ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity<0.188ng/ml
  • Intended UseHuman MTBP ELISA Kit allows for the in vitro quantitative determination of MTBP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-MTBP antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-MTBP antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the MTBP amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of MTBP can be calculated.
  • Human Mdm2-binding protein Protein information
  • Uniprot ID MTBP_HUMAN
  • Uniprot AC Q96DY7; B4DUR5; Q9HA89;
  • UniGene Hs.657656;
  • GeneID 27085
  • KEGG hsa:27085;
  • Human Mdm2-binding protein Protein SEQUENCE
  • SEQUENCE 904 AA; 102193 MW; 753DF4A98AB62E4F CRC64;

    MDRYLLLVIW GEGKFPSAAS REAEHGPEVS SGEGTENQPD FTAANVYHLL

    KRSISASINP EDSTFPACSV GGIPGSKKWF FAVQAIYGFY QFCSSDWQEI

    HFDTEKDKIE DVLQTNIEEC LGAVECFEEE DSNSRESLSL ADLYEEAAEN

    LHQLSDKLPA PGRAMVDIIL LLSDKDPPKL KDYLPTVGAL KHLREWYSAK

    ITIAGNHCEI NCQKIAEYLS ANVVSLEDLR NVIDSKELWR GKIQIWERKF

    GFEISFPEFC LKGVTLKNFS TSNLNTDFLA KKIIPSKDKN ILPKVFHYYG

    PALEFVQMIK LSDLPSCYMS DIEFELGLTN STKQNSVLLL EQISSLCSKV

    GALFVLPCTI SNILIPPPNQ LSSRKWKEYI AKKPKTISVP DVEVKGECSS

    YYLLLQGNGN RRCKATLIHS ANQINGSFAL NLIHGKMKTK TEEAKLSFPF

    DLLSLPHFSG EQIVQREKQL ANVQVLALEE CLKRRKLAKQ PETVSVAELK

    SLLVLTRKHF LDYFDAVIPK MILRKMDKIK TFNILNDFSP VEPNSSSLME

    TNPLEWPERH VLQNLETFEK TKQKMRTGSL PHSSEQLLGH KEGPRDSITL

    LDAKELLKYF TSDGLPIGDL QPLPIQKGEK TFVLTPELSP GKLQVLPFEK

    ASVCHYHGIE YCLDDRKALE RDGGFSELQS RLIRYETQTT CTRESFPVPT

    VLSPLPSPVV SSDPGSVPDG EVLQNELRTE VSRLKRRSKD LNCLYPRKRL

    VKSESSESLL SQTTGNSNHY HHHVTSRKPQ TERSLPVTCP LVPIPSCETP

    KLATKTSSGQ KSMHESKTSR QIKESRSQKH TRILKEVVTE TLKKHSITET

    HECFTACSQR LFEISKFYLK DLKTSRGLFE EMKKTANNNA VQVIDWVLEK

    TSKK

  • UCSC uc003ypc.3; human. [Q96DY7-1];



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