Human MICA ELISA Kit

Alternative name
Human MIC-A ELISA Kit;Human PERB11.1 ELISA Kit;Human MHC class I polypeptide-related sequence A ELISA Kit;Human HLA class I antigen ELISA Kit;Human MHC class I chain-related protein A ELISA Kit;Human MHC class I related chain A ELISA Kit;Human MHC class I related sequence A ELISA Kit;Human stress inducible class I homolog ELISA Kit;Human truncated MHC class I polypeptide-related sequence A ELISA Kit;
Catalog
E024418
species
Human
GeneMICA
Standard Curve
Other Species Mouse MICA ELISA Kit
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 pg/mL.
Intended UseHuman MICA ELISA Kit allows for the in vitro quantitative determination of MICA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyImmunology
Product Description
specificalFor samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate INTENDED USE This MICA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human MICA. This ELISA kit for research use only, not for therapeutic or diagnostic applications! PRINCIPLE OF THE ASSAY MICA ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MICA antibody and an MICA-HRP conjugate. The assay sample and buffer are incubated together with MICA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MICA concentration since MICA from samples and MICA-HRP conjugate compete for the anti-MICA antibody binding site. Since the number of sites is limited, as more sites are occupied by MICA from the sample, fewer sites are left to bind MICA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MICA concentration in each sample is interpolated from this standard curve.
Human MHC class I polypeptide-related sequence A Protein information
Uniprot ID MICA_HUMAN
Uniprot AC Q9TQL1; Q9TQL2; Q9TQL3; Q9TQL4; Q9UE04; Q9UE05; Q9UE06; Q9UE11;
UniGene Hs.130838;
GeneID 100507436
KEGG hsa:100507436;
Human MHC class I polypeptide-related sequence A Protein SEQUENCE
SEQUENCE 383 AA; 42915 MW; 1FE084813DDA13A8 CRC64;
MGLGPVFLLL AGIFPFAPPG AAAEPHSLRY NLTVLSWDGS VQSGFLTEVH
LDGQPFLRCD RQKCRAKPQG QWAEDVLGNK TWDRETRDLT GNGKDLRMTL
AHIKDQKEGL HSLQEIRVCE IHEDNSTRSS QHFYYDGELF LSQNLETKEW
TMPQSSRAQT LAMNVRNFLK EDAMKTKTHY HAMHADCLQE LRRYLKSGVV
LRRTVPPMVN VTRSEASEGN ITVTCRASGF YPWNITLSWR QDGVSLSHDT
QQWGDVLPDG NGTYQTWVAT RICQGEEQRF TCYMEHSGNH STHPVPSGKV
LVLQSHWQTF HVSAVAAAAI FVIIIFYVRC CKKKTSAAEG PELVSLQVLD
QHPVGTSDHR DATQLGFQPL MSDLGSTGST EGA
UCSC uc011ind.3; human. [Q29983-1];

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Human MICA ELISA Kit