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Alternative name
Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha ELISA KIT; Phosphoinositide 3-kinase-C2-alphaPIK3C2A ELISA KIT; PI3K-C2-alpha ELISA KIT; PtdIns-3-kinase C2 subunit alpha ELISA KIT
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Catalog
E029124
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species
Human
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GenePI3K2alpha
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity<1.875mU/ml
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Intended UseHuman PI3K2alpha ELISA Kit allows for the in vitro quantitative determination of PI3K2alpha , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human PI3K2alpha antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human PI3K2alpha antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human PI3K2alpha amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human PI3K2alpha can be calculated.
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