Human HSPB3 ELISA Kit

Alternative name
Human HspB3 ELISA Kit;Human heat shock 17 kDa protein ELISA Kit;Human HSP 17 ELISA Kit;Human protein 3 ELISA Kit;Human HSP27 ELISA Kit;Human HSPL27 ELISA Kit;Human DHMN2C ELISA Kit;Human HMN2C ELISA Kit;Human heat shock protein family B (small) member 3 ELISA Kit;Human heat shock protein beta-3 ELISA Kit;Human heat shock 27kDa protein 3 ELISA Kit;
Catalog
E017936
species
Human
GeneHSPB3
SpecificityThis assay has high sensitivity and excellent specificity for detection of human HSPB3. No significant cross-reactivity or interference between human HSPB3 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human HSPB3 is typically less than 0.195 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the m
Intended UseHuman HSPB3 ELISA Kit allows for the in vitro quantitative determination of HSPB3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HSPB3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HSPB3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HSPB3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HSPB3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Heat shock protein beta-3 Protein information
Uniprot ID HSPB3_HUMAN
Uniprot AC Q12988;
UniGene Hs.41707;
GeneID 8988
KEGG hsa:8988;
Human Heat shock protein beta-3 Protein SEQUENCE
SEQUENCE 150 AA; 16966 MW; CE5A4DF34CD38715 CRC64;
MAKIILRHLI EIPVRYQEEF EARGLEDCRL DHALYALPGP TIVDLRKTRA
AQSPPVDSAA ETPPREGKSH FQILLDVVQF LPEDIIIQTF EGWLLIKAQH
GTRMDEHGFI SRSFTRQYKL PDGVEIKDLS AVLCHDGILV VEVKDPVGTK

Human HSPB3 ELISA Kit

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