Human HCRT ELISA Kit

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  • Alternative name

    Human Hypocretin ELISA Kit;Human Hcrt ELISA Kit;Human Hypocretin-1 ELISA Kit;Human Hcrt1 ELISA Kit;Human Hypocretin-2 ELISA Kit;Human Hcrt2 ELISA Kit;Human OX ELISA Kit;Human PPORX ELISA Kit;Human PPOX ELISA Kit;Human NRCLP1 ELISA Kit;Human hypocretin neuropeptide precursor ELISA Kit;Human orexin ELISA Kit;Human hypocretin (orexin) neuropeptide ELISA Kit;Human prepro-orexin ELISA Kit;

  • Catalog
    E027745
  • species
    Human
  • GeneHCRT
  • Other Species Mouse Hcrt ELISA KitBovine HCRT ELISA KitCanine HCRT ELISA KitPorcine HCRT ELISA KitRat Hcrt ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity< 9.4pg/ml
  • Intended UseHuman HCRT ELISA Kit allows for the in vitro quantitative determination of HCRT , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- HCRT antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- HCRT antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the HCRT amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of HCRT can be calculated.
  • Human Orexin-B Protein information
  • Uniprot ID OREX_HUMAN
  • Uniprot AC O43612;
  • UniGene Hs.158348;
  • GeneID 3060
  • KEGG hsa:3060;
  • Human Orexin-B Protein SEQUENCE
  • SEQUENCE 131 AA; 13363 MW; 139D9C33E39E4EF1 CRC64;

    MNLPSTKVSW AAVTLLLLLL LLPPALLSSG AAAQPLPDCC RQKTCSCRLY

    ELLHGAGNHA AGILTLGKRR SGPPGLQGRL QRLLQASGNH AAGILTMGRR

    AGAEPAPRPC LGRRCSAPAA ASVAPGGQSG I

  • UCSC uc002hzc.1; human.;



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