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Mouse NET ELISA Kit

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  • Alternative name

    Sodium-dependent noradrenaline transporter ELISA KIT; Norepinephrine transporter ELISA KIT; NET ELISA KIT; Solute carrier family 6 member 2SLC6A2 ELISA KIT; NAT1 ELISA KIT; NET1 ELISA KIT; SLC6A5 ELISA KIT; NET ELISA KIT
  • Catalog
    E061811
  • species
    Mouse
  • GeneNET
  • Standard CurveMouse NET ELISA Kit
  • Other Species Human NET ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Norepinephrine Transporter (NET). No significant cross-reactivity or interference between Norepinephrine Transporter (NET) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.062ng/mL.
  • Intended UseMouse NET ELISA Kit allows for the in vitro quantitative determination of NET , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Norepinephrine Transporter (NET). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Norepinephrine Transporter (NET). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Norepinephrine Transporter (NET), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Norepinephrine Transporter (NET) in the samples is then determined by comparing the O.D. of the samples to the standard curve.



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